中英文说明丨艾美捷InVivoMAb 抗小鼠NK1.1

The PK136 monoclonal antibody reacts with mouse NK1.1 also known as CD161b/CD161c, KLRB1, NKR-P1A and Ly-55. NK1.1 is a type II integral membrane glycoprotein with a C-type lectin domain and is encoded by the Klrb1c/NKR-P1C gene. NK1.1 plays roles in NK cell activation and differentiation, IFN-γ production, cytotoxic granule release, and is thought to be involved in the generation of Th2 cells. NK1.1 is predominantly expressed as a disulfide-linked homodimer on NK cells however, it is also expressed on NK-T cells, a rare population of T lymphocytes. NK 1.1 is only expressed by C57BL/6, FVB/N, and NZB strains of mice and not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129 strains.

Isotype Mouse IgG2a, κ

Recommended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Mouse spleen and bone marrow cells enriched for NK1+ cells

Reported Applications in vivo NK cell depletion

Flow cytometry

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein A

RRID AB_1107737

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

PK136单克隆抗体与小鼠NK1.1反应，也称为CD161b / CD161c，KLRB1，NKR-P1A和Ly-55。NK1.1是一种具有C型凝集素结构域的II型整合膜糖蛋白，由Klrb1c/NKR-P1C基因编码。NK1.1在NK细胞活化和分化，IFN-γ产生，细胞毒性颗粒释放中起作用，并被认为参与Th2细胞的产生。NK1.1主要在NK细胞上表达为二硫键连接的同源二聚体，然而，它也在NK-T细胞上表达，NK-T细胞是一种罕见的T淋巴细胞群。NK 1.1仅由小鼠的C57BL / 6，FVB / N和NZB菌株表达，而不是AKR，BALB / c，CBA / J，C3H，DBA / 1，DBA / 2，NOD，SJL和129株表达。

艾美捷InVivoMAb抗小鼠NK1.1#BE0036基本参数：

同种型 小鼠IgG2a， κ

推荐的同种型对照 体内MAb小鼠IgG2a同种型对照，特异性未知

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 富含NK1+细胞的小鼠脾脏和骨髓细胞

报告的应用程序 体内NK细胞去除

流式细胞术

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白A

里德 AB_1107737

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

InVivoMAb抗小鼠NK1.1部分文献引用：

in vivo NK cell depletion

Ghasemi, R., et al. (2016). "Selective targeting of IL-2 to NKG2D bearing cells for improved immunotherapy" Nat Commun 7: 12878.

in vivo NK cell depletion, Flow Cytometry

Maltez, V. I., et al. (2015). "Inflammasomes Coordinate Pyroptosis and Natural Killer Cell Cytotoxicity to Clear Infection by a Ubiquitous Environmental Bacterium" Immunity 43(5): 987-997.

in vivo NK cell depletion, Flow Cytometry

Wensveen, F. M., et al. (2015). "NK cells link obesity-induced adipose stress to inflammation and insulin resistance" Nat Immunol 16(4): 376-385.

in vivo NK cell depletion

Yamada, D. H., et al. (2015). "Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection" Immunity 42(2): 379-390.