中英文说明丨艾美捷InVivoMAb 抗小鼠 IL-4介绍

The 11B11 monoclonal antibody reacts with mouse IL-4 (interleukin-4) a multifunctional 14 kDa cytokine. IL-4 is expressed primarily by activated Th2 cells and NK cells, and at lower levels by mast cells, and basophils. IL-4 signals through the IL-4Rα. Upon receptor binding IL-4 stimulates activated B and T lymphocyte proliferation, and the differentiation of B cells into plasma cells. It also induces B cell class switching to IgE, and up-regulates MHC class II production while decreasing the production of Th1 cells, macrophages, IFNγ, and dendritic cell IL-12. Like other Th2 associated cytokines, IL-4 is involved in the airway inflammation observed in the lungs of patients with allergic asthma. The 11B11 monoclonal antibody has been shown to neutralize the bioactivity of natural or recombinant IL-4.

Isotype Rat IgG1, κ

Recommended Isotype Control(s) InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Partially purified native mouse IL-4

Reported Applications in vivo IL-4 neutralization

in vitro IL-4 neutralization

in vivo IL-4 receptor stimulation (as a complex with IL-4)

Flow cytometry

Western blot

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein G

RRID AB_1107707

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

11B11单克隆抗体与小鼠IL-4（白细胞介素-4）反应，这是一种多功能14kDa细胞因子。IL-4主要由活化的Th2细胞和NK细胞表达，在较低水平上由肥大细胞和嗜碱性粒细胞表达。IL-4通过IL-4Rα发出信号。受体结合后，IL-4刺激活化的B和T淋巴细胞增殖，以及B细胞分化为浆细胞。它还诱导B细胞类切换到IgE，并上调MHC II类的产生，同时减少Th1细胞，巨噬细胞，IFNγ和树突状细胞IL-12的产生。与其他Th2相关细胞因子一样，IL-4参与过敏性哮喘患者肺部观察到的气道炎症。11B11单克隆抗体已被证明可以中和天然或重组IL-4的生物活性。

艾美捷InVivoMAb 抗小鼠 IL-4#BE0045基本参数：

同种型 大鼠IgG1， κ

推荐的同种型对照 体内MAb大鼠IgG1同种型对照，抗辣根过氧化物酶

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 部分纯化的天然小鼠IL-4

报告的应用程序 体内IL-4体外中和IL-4体内中

和

IL-4受体刺激（与IL-4复合）

流式细胞术

蛋白质印迹

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白 G

里德 AB_1107707

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

InVivoMAb抗小鼠IL-4部分文献引用：

in vitro IL-4 neutralization

Hill, E. V., et al. (2015). "Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter" Eur J Immunol 45(4): 1103-1115.

in vitro IL-4 neutralization

Choi, Y. S., et al. (2015). "LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6" Nat Immunol 16(9): 980-990.

in vitro IL-4 neutralization

Kim, Y. U., et al. (2015). "Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells" PLoS One 10(3): e0120294.

in vitro IL-4 neutralization

Hou, L., et al. (2015). "The protease cathepsin L regulates Th17 cell differentiation" J Autoimmun. S 0896-8411(15): 30024-X.