中英文说明书丨艾美捷FD NeuroSilver退化神经元银染

FD NeuroSilver™ Kit II is designed for the detection of degenerating neurons in fixed tissue sections of the central nervous system from experimental animals. The principle of this kit is based on the findings that some components of the neurons undergoing degeneration, such as lysosomes, axons, and terminals, become particularly argyrophilic. Under certain conditions, these cellular elements bind to the silver ions with high affinity. Upon reduction, the silver ions form metallic grains that are visible under a light or electron microscope.

FD NeuroSilver™ Kit II has been used widely in animal studies under various experimental conditions. This kit has proven extremely specific and sensitive for the detection of degenerating neuronal somata, axons, and terminals in both the brain and the spinal cord (cf. photo samples). It is particularly useful for the detection of small numbers of degenerating neurons that may not be demonstrable with routine histopathological techniques.

FD NeuroSilver™ Kit II has also been proven to be very sensitive and reliable for the detection of amyloid plaques in the brain of transgenic mice (cf. photo samples). In addition, this kit may be used for demonstrating neurodegeneration and/or amyloid plaques in tissue sections that have been processed for immunohistochemistry (cf. photo samples). The procedure of FD NeuroSilver™ Kit II takes approximately 1 hour.

Kit contents:

Solution A                                      500 ml

Solution B                                      500 ml

Solution C                                      500 ml x 2

Solution D                                      500 ml

Solution E                                       2 ml

Solution F                                       3 ml

Solution G (10X)                             500 ml

Glass Specimen Retriever                2

Natural hair paintbrush                     1

User Manual                                     1

艾美捷FD NeuroTech FD NeuroSilver退化神经元银染试剂盒II（#PK301）设计用于检测实验动物中枢神经系统固定组织切片中的退化神经元。该试剂盒的原理是基于以下发现：经历退化的神经元的某些成分，如溶酶体、轴突和末端，变得特别嗜银。在某些条件下，这些细胞元素以高亲和力与银离子结合。还原后，银离子形成金属颗粒，在光学或电子显微镜下可见。

FD NeuroSilver退化神经元银染试剂盒II已广泛用于各种实验条件下的动物研究。该试剂盒已被证明对检测大脑和脊髓中的退化神经元体瘤、轴突和末端具有极-高的特异性和灵敏度。它对于检测少量退化神经元特别有用，这些神经元可能无法通过常规组织病理学技术证明。

FD NeuroSilver退化神经元银染试剂盒II也被证明对检测转基因小鼠大脑中的淀粉样斑块非常敏感和可靠（参见照片样品）。此外，该试剂盒可用于显示已处理用于免疫组织化学的组织切片中的神经变性和/或淀粉样斑块。FD神经银™试剂盒II的过程大约需要1小时。