中英文说明书丨艾美捷FD NeuroApap神经元TUNEL凋亡检测

FD NeuroApop™ Kit is specifically designed for the detection of neuronal apoptosis in tissue sections from the central nervous system based on the principle of in situ DNA nick-end labeling (TUNEL) technique. The assay uses terminal deoxynucleotidyl transferase to catalyze the incorporation of biotinylated deoxyuridines onto the free 3′-hydroxyl termini of DNA fragments, which are considered one of the most characteristic features of apoptosis. The integrated biotins are amplified and visualized with the avidin-biotin-complex (ABC) method4, enabling light microscopic identification.

The reagents and procedure of FD NeuroApop™ Kit have been optimized to achieve a high degree of both specificity and sensitivity for detecting apoptotic neurons with the lowest background. This kit can be used with frozen and paraffin sections, as well as cultured cells (cf. photo samples below).  The procedure of the kit takes approximately 4 hours.

Kit contents:

Part I (Store at -20°C)

Digestive Enzyme                                    2 ml x 4

Reaction Solution A                                 2 ml x 2

Reaction Solution B                                 85 µl

Reaction Solution C                                 60 µl

Chromogen Solution                                20 ml

Part II (Store at 4°C)

Equilibration Buffer                                   20 ml

Detection Reagent                                    5 ml

10x Phosphate-Buffered Saline             250 ml x 2

艾美捷FD NeuroTech FD NeuroApap 神经元TUNEL凋亡检测试剂盒（#PK201）专为检测中枢神经系统组织切片中的神经元凋亡而设计，基于原位DNA切口末端标记（TUNEL）技术的原理。该测定使用末端脱氧核苷酸转移酶催化生物-素化脱氧尿苷掺入DNA片段的游离3′-羟基末端，这被认为是细胞凋亡比较典型的特征之一 2, 3.整合的生物-素通过亲和素-生物-素复合物（ABC）方法进行扩增和可视化4，可实现光学显微镜识别。

FD NeuroApap 神经元TUNEL凋亡检测试剂盒（#PK201）的试剂和程序已经过优化，在检测具有极低背景的凋亡神经元时实现了高度的特异性和灵敏度。该试剂盒可用于冷冻和石蜡切片以及培养细胞（参见下面的照片样品）。该套件的过程大约需要 4 小时。

FD NeuroApap 神经元TUNEL凋亡检测试剂盒文献引用：

1.Gavrieli Y, Sherman Y, and Ben-Sasson SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501.

2.Wyllie AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 284:555-6.

3.Arends MJ, Morris RG, and Wyllie AH. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol. 136:593-608.

4.Hsu SM, Raine L, and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-80.