血管紧张素ⅡELISA试剂盒——比色、竞争性免疫测定

ADI-900-204血管紧张素ⅡELISA试剂盒：

Sensitivity: 4.6 pg/ml (range 3.9-10,000 pg/ml)

Assay Time: 3.5 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of species independent Angiotensin II in plasma and serum samples.

Wavelength: 450 nm

Species reactivity: Species independent

Crossreactivity: Angiotensin II (100%), Angiotensin A (100%), Angiotensin III (100%), Angiotensin IV (100%), Angiotensin I (0.319%), Angiotensin (1-9) (0.103%), LVV-hemorphin 7 (0.067%), Angiotensin (1-7) (0.053%), Bradykinin (0.022%)

Quantity: 1 x 96-well plate

Use/Stability: Store all components at +4ºC, except Standard at -20ºC.

Shipping: Shipped on Blue Ice

Contents: Microtiter Plate, Conjugate, Antibody, Assay Buffer 16, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Streptavidin-HRP

Scientific Background: Angiotensins are small peptides derived from angiotensinogen. Several of the known angiotensins are established endocrine effectors in the regulation of blood pressure, but they are also known to have other functions locally, in several organs and tissues (paracrine) and at the cellular level (autocrine / intracrine). Angiotensin I (DRVYIHPFHL) has no known effector function but it is an immediate precursor of Angiotensin II (DRVYIHPF). Angiotensin II binds AT1 receptors, which promote vasoconstriction, sodium retention, release of aldosterone, release of Arg-vasopressin, cell proliferation, inflammation, fibrosis, anxiety, and cardiac hypertrophy. Angiotensin A (ARVYIHPF), and Angiotensin III (RVYIHPF), also bind AT1 receptors. Angiotensin (1-7) (DRVYIHP) binds a different receptor called MAS-1 which has opposite effects (vasodilation, natriuresis, antiproliferation, NO release, PGE release, and apoptosis). Angiotensin IV (VYIHPF) binds yet another receptor called AT4 (IRAP), which promotes increase of blood flow, angiogenesis, and natriuresis, and which has also been implicated in memory formation and in the pathogenesis of Alzheimer’s disease. The peptide LVV-hemorphin-7 (LVVYPWTQRF), which is not an angiotensin, also binds the AT4 receptor. There are other angiotensin peptides that have been identified, including Ang (1-9) (DRVYIHPFH), Ang (1-12) (DRVYIHPFHLVI), Ang V(3-7) (VYIHP), as well as several other shorter peptides that have undetermined functions.

艾美捷ADI-900-204 血管紧张素ⅡELISA试剂盒，高灵敏度ELISA，检测量低至4.6pg/ml，该试剂盒是一种比色、竞争性免疫测定试剂盒，3.5小时内即可获得结果。

ADI-900-204血管紧张素ⅡELISA试剂盒化学性质：

灵敏度：4.6 pg/ml（范围3.9-10000 pg/ml）

化验时间：3.5小时

应用：ELISA，比色检测

应用说明：用于定量测定血浆和血清样品中的种类无关的血管紧张素II。

波长：450 nm

物种反应性：物种无关

交叉反应性：血管紧张素II（100%），血管紧张素A（100%）、血管紧张素III（100%）和血管紧张素IV（100%）

数量：1 x 96孔板

使用/稳定性：将所有组件储存在+4ºC，标准组件除外，储存在-20ºC。

运输：蓝冰运输

内容：微量滴定板、缀合物、抗体、测定缓冲液16、洗涤缓冲液浓缩液、标准品、TMB底物、停止溶液2、链霉亲和素HRP

血管紧张素ⅡELISA试剂盒文献参考：

氯沙坦和维生su-D抑制散发性癌症条件Apc缺失小鼠模型中结肠癌的发展：U.Dougherty等人。；癌症预防。Res.（Phila.）12433（2019）；

microRNA-25表达的改变通过肾素分泌调节心脏功能：H.Li等人。；实验细胞研究395119（2018）；

与葡萄糖一起摄入全蛋或蛋清可防止餐后高血糖引起的氧化应激和精-氨-酸代谢失调，并改善糖尿病前期男性的血管内皮功能：J.D.McDonald等人。；《营养学杂志》。120901（2018），应用：人血浆；

硫化氢减弱肾素-血管紧张素和醛固酮病理信号，以保护肾功能并改善心脏的运动耐受性：Z.Li，et al。；JACC基础翻译。科学。3，796（2018），应用：使用小鼠肾脏的ELISA