艾美捷植物染色质免疫沉淀(ChIP)试剂盒，不到六小时！

Amyjet Plant chromatin immunoprecipitation (ChIP) Kit # P-2014-24 can effectively detect protein DNA interaction in plants in vitro, which is easy to operate. The operation process can obtain better results than other kits in less than six hours. chromatin obtained from the kit can be used for qualitative and quantitative PCR, southern blotting and DNA microarray analysis

The plant chromatin immunoprecipitation (ChIP) kit provides all the reagents needed for the chromatin immunoprecipitation reaction of plant cell samples. In addition, there is also a ChIP grade dimethyl histone H3-K9 antibody and a negative control antibody (IgG of normal mice) in the kit. The chromatin extracted from the cell samples is properly interrupted, Then it is added to the micropores to undergo an immune reaction with the antibodies adsorbed on their surface. The DNA specifically bound to the micropores is released from the antibody capture protein DNA complex, flipped over, and purified through our company's specially designed high-speed centrifuge column. The eluted DNA can be used for various subsequent analyses

艾美捷植物染色质免疫沉淀(ChIP)试剂盒#P-2014-24可以有效进行体外检测植物中蛋白质-DNA相互作用，操作方便.操作过程不到六小时就可以获得比其它试剂盒更好的结果.试剂盒获得的染色质可用来进行定性和定量PCR、southern 印迹以及DNA微阵列分析.

植物染色质免疫沉淀(ChIP)试剂盒提供了对植物细胞样品进行染色质免疫沉淀反应所需的所有试剂.并且，试剂盒中还有一种ChIP级二甲基组蛋白H3-K9抗体和一种阴性对照抗体（正常小鼠的IgG）.从细胞样品中提取出来的染色质进行适当的打断，然后加入到微孔中与其表面上吸附的抗体发生免疫反应.特异性结合到微孔上的DNA从抗体-捕获蛋白-DNA复合物上释放出来，翻转后，通过本公司专门设计的高速离心柱纯化.洗脱下来的DNA可用于随后的各种分析.

该植物染色质免疫沉淀(ChIP)试剂盒组成：

Components 24 readions     48 regdions  

P-2014-24       P-2014-48  

CP1 (Wash Buffer) 28ml        2x28ml  

CP2  (Antibody Buffer)     15ml   30ml

CP3C (5X Lysis Buffer )      12ml    24ml

CP3D (Lysis Buffer II) 3ml   6ml  

CP3E (Lysis Buffer II) 2ml   4ml  

CP3F (Lysis Buffer M) 1.5 ml  5ml  

CP4 (ChP  Dilution Buffer) 2ml  6ml  

CP5 (DNA Release Buffer) 2ml  2x2ml  

CP6( Reverse Buffer) 2ml   2x2ml  

CP7(Binding  Buffer) 5ml  8  ml  

CP8 (Elution Buffer) 0.6ml   1.2ml  

Protease Inhibitor Cocktail (100X)* 25μl  50μl  

Non-immune  lgG  (1mg/m)* 10μ 10μ  

Anti-Dimethyl  H3-K9 (1 mg/ml)* * 5μl   8μl  

Proteinase K (10 mg/ml)* 25μl    50μl  

8-Well Assay Strips (with frame) 3  6

8-Well Srip Caps 3 6

F-Spin  Column 30  50  

F-Collection Tube 30   50  

*Spin the solution down to the boffom before use.  

该植物染色质免疫沉淀(ChIP)试剂盒特点：

1、市场上同类产品中较为快捷的试剂盒，整个处理过程不到6小时；

2、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；

3、附有DNA纯化柱子：大量减少不必要的重复劳动；

4、操作简便、结果可靠、统一的分析条件；