中文名称:辣根过氧化物酶HRP标记亲和纯化山羊抗兔IgG(H+L)二抗 现货
英文名字:Horseradish Peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
编号:111-035-003
规格:2ml
应用类型:WB(1:10000 - 1:200000)、ELISA(1:5000 - 1:100000) IHC(1:500 - 1:5000)
免疫原:兔IgG
来源宿主:山羊
反应性:该山羊抗兔二抗与兔IgG分子有特异性反应,与其它兔免疫球蛋白(包括IgM、IgE、IgD、IgA等)的轻链有交叉反应。该二抗与血清内非免疫球蛋白无交叉反应,但可能与其它种属的免疫球蛋白有交叉反应。
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Physical State: Freeze-dried solid
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
对正常血清进行脂质提取以提高清晰度,用含有叠-氮-化-钠的磷酸盐缓冲盐水(PBS)透析,并冷冻干燥。强烈推荐在PBS中稀释至5%(v/v)的正常血清作为阻断试剂,以减少非特异性、保守序列和/或Fc受体结合的背景。作为添加第一抗体之前的单独培养步骤,用来自与标记抗体相同宿主的稀释的正常血清获得非常好的结果。
艾美捷HRP标记亲和纯化山羊抗兔IgG(H+L)二抗#111-035-003,基于免疫电泳和/或ELISA,抗体与全分子兔IgG反应。它还与其他兔免疫球蛋白的轻链发生反应。未检测到针对非免疫球蛋白血清蛋白的抗体。抗体可以与来自其他物种的免疫球蛋白发生交叉反应。
通过免疫亲和层析从抗血清中分离出完整的IgG抗体分子。它们具有通过二硫键连接在一起的Fc部分和两个抗原结合Fab部分,因此它们是二价的。据报道,平均分子量为约160kDa。整个IgG形式的抗体适用于大多数免疫检测程序,并且是极-具成本效益的。
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