产品名称 | 英文名称 | 规格 | 价格 |
| 250g | 电询 |
用于金黄色网葡萄球菌增菌
7.5%氯化钠肉汤管现货供应实验内容
1.称量→溶化→调pH→过滤→分装→加塞→包扎→灭菌→无菌检查
2.干热灭菌:装入待灭菌物品→升温→恒温→降温→开箱取物
3.高压蒸汽灭菌:加水→装物品→加盖→加热→排冷空气→加压→恒压→降压回零→排汽→取物→无菌检查
4.过滤除菌:组装灭菌→连接→压滤→无菌检查→清洗灭菌
保存条件:
培养基由于配制的原料不同,使用要求不同,而贮存保管方面也稍有不同。一般培养基在受热、吸潮后,易被细菌污染或分解变质,因此一般培养基必须防潮、避光、阴凉处保存。对一些需严格灭菌的培养基(如组织培养基),较长时间的贮存,必须放在2~6℃的冰箱内。由于液体培养基不易*保管,现在均改制成粉末。
的步骤:
(一)准确称量试剂:根据不同的菌类和用途,选择适宜的培养基,培养基所需试剂必须纯净。
(二)校正pH值:将称量好的培养基各种成分放入容器内,标记划线,加热溶解,补充水分,测定酸碱度,常用pH6.8~8.0的精密试纸或酸度计测定。用1N NaOH和1N HCl调节pH值到适宜范围内。
(三)过滤:将玻璃漏斗置铁架上,再用纱布夹棉花或用滤纸放在漏斗中,将上述培养基倒入其中过滤至透明。
(四)分装:将过滤后的培养基分装于中试管或三角瓶内(试管内每支装5mL;三角瓶中装100~150ml),塞好棉塞用牛皮纸包扎好,准备灭菌。
(五)灭菌:培养基的灭菌,常用高压蒸汽灭菌法。一般微生物的营养细胞在水中煮沸后即被杀死,但细菌的芽胞有较强的抗热性,须经高压蒸汽灭菌才能达到*灭菌的目的。根据蒸汽温度随压力升高而上升的原理,即压力越大,蒸汽温度就越高。因此,在同一温度条件下,采用高压蒸汽灭菌比干热灭菌法效果要好。而且在湿热情况下,菌体吸收水分后,其蛋白质易于凝固变性,因为蒸汽的穿透力强,杀菌效果好。
都经过国家质量体系安全验证,从到销售都经过严格把关确保质量合格后方可出售,包装完好,内附检验报告。
factor,ESF ELISA Kit 96T/48T
大鼠血小板因子4(PF-4/CXCL4)ELISA Kit,48T/96T M00571 Human TNF related activation induced cytokine,TRANCE ELISA Kit 96T/48T
血小板因子4(PF-4/CXCL4)ELISA Kit,48T/96T M00572 Human Epithelial Cell Adhesion Molecule,Ep-CAM ELISA Kit 96T/48T
人α半乳糖苷酶(αGAL)ELISA Kit,48T/96T M00573 Human Alternative macrophage activation-associated CC chemokine 1,AmAC-1 ELISA Kit 96T/48T
人基质金属蛋白酶组织抑制因子1(TIMP-1)ELISA Kit,48T/96T M00574 Human soluble vascular endothelial growth factor receptor-2,VEGFR-2/sFLK-1 ELISA Kit 96T/48T
人α甘露糖苷酶(α Manase)ELISA Kit,48T/96T M00575 Human thymic stromal lymphopoietin,TSLP ELISA Kit 96T/48T
小鼠α甘露糖苷酶(α Manase)ELISA Kit,48T/96T M00576 human soluble cluster of differeiation 28,sCD28 ELISA Kit 96T/48T
人Ⅲ型胶原(Col Ⅲ)ELISA Kit,48T/96T M00577 Human thymus activation regulated chemokine,TARC ELISA Kit 96T/48T
小鼠Ⅲ型胶原(Col Ⅲ)ELISA Kit,48T/96T M00578 Human Neural cell adhesion molecule ligand 1,NCAM-L1 ELISA Kit 96T/48T
猪Ⅲ型胶原(Col Ⅲ)ELISA Kit,48T/96T M00579 Human soluble Factor-related Apoptosis ligand,sFASL/Apo-1 ELISA Kit 96T/48T
大鼠Ⅲ型胶原(Col Ⅲ)ELISA Kit,48T/96T M00580 Human inhibitor of apoptosis,IAP ELISA Kit 96T/48T
Ⅲ型胶原(Col Ⅲ)ELISA Kit,48T/96T M00581 Human monocyte ierferon gamma inducing factor,MIGF ELISA Kit 96T/48T
鸡α葡萄糖苷酶(α-glucosidase)ELISA Kit,48T/96T M00582 Human Ierferon inducible T-cell Chemoattracta,I-TAC ELISA Kit 96T/48T
人α葡萄糖苷酶(α-glucosidase)ELISA Kit,48T/96T
PPAR Gamma (peroxisome proliferator-activated receptor-gamma)
PP2Ac
Runx3
RRAD (Ras-related associated with diabetes )
SDF-1/CXCL12 (stromal cell derived factor-1)
shank1(SH3 and multiple anleyrin repead domins protein 1)
slc22A17 (solute carrier famili 22)
SLC24A5 (solute carrier family 24, member 5)
solute carrier family 1
SLC6A4 (solute carrier family 6)
Survivin
SSA/RO (ribonucleoprotein complex,RNP)
SSB/La
Smad2(Mothers against decapeaplegic homolog 2)
SPAG5/map126
SP-A (pulmonary surfacta-associated glycoprotein A)
SYVN1(synovial apoptosis inhibitor 1)
SYK (Spleen tyrosine kinase)
Tau protein
Thy-1/CD90
Thioster-coaining protein 1
TIGAR humen (TP53- induced glycolysis and apoptosis-regulator )
TIMP-4(Tissue Inhibitor of Metalloproteinase-4)