ELISA是以免疫学反应为基础，将抗原、抗体体的特异性反应与酶对底物的高效催化作用相结合起来的一种敏感性很高的试验技术。
小鼠可溶性瘦素受体 sLR 是北京方程生物公司的优势产品，2017年买小鼠可溶性瘦素受体 sLR 送好礼*活动火热进行中......
Pig Thy1 Cell Surface Antigen (Thy1) ELISA
ELISA实验小知识：ELISA检测试剂盒标本的稀释原则：首先通过文献检索的方式了解待测样本的大致含量，确定适当的稀释倍数。只有稀释至标准曲线的范围内，检测的结果才是准确的。稀释的过程中，应做好详细的记录。zui后计算浓度时，稀释了“N”倍，标本的浓度应再乘以“N”。
Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
猪*Ⅴ(F5)检测盒Pig Coagulation Factor V (F5) ELISA
人白介素20(IL20)测定盒Human Interleukin 20 (IL20) ELISA
人Ⅹ型胶原(COL10)测定盒Human Collagen Type X (COL10) ELISA
猪*羟化酶(TH)检测盒Pig Tyrosine Hydroxylase (TH) ELISA
猪丝裂原激活蛋白激酶12(MAPK12)检测盒Pig Mitogen Activated Protein Kinase 12 (MAPK12) ELISA
猪氨基端前脑钠素(NT-ProBNP)检测盒Pig N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA
猪生长分化因子1(GDF1)检测盒Pig Growth Differentiation Factor 1 (GDF1) ELISA
人Toll样受体9(TLR9)测定盒Human Toll Like Receptor 9 (TLR9) ELISA
猪游离脂肪酸受体3(FFAR3)检测盒Pig Free Fatty Acid Receptor 3 (FFAR3) ELISA
猪半*蛋白酶抑制剂B(CSTB)检测盒Pig Cystatin B (CSTB) ELISA
猪醛固酮(ALD)检测盒Pig Aldosterone (ALD) ELISA
猪抗利尿激素(ADH)检测盒Pig Antidiuretic Hormone (ADH) ELISA
