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Pig D-Dimer (D2D) ELISA
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Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
人丝聚蛋白(FLG)测定盒Human Filaggrin (FLG) ELISA
猪腺苷激酶(ADK)检测盒Pig Adenosine Kinase (ADK) ELISA
猪β-羟丁酸(OHβ)检测盒Pig Beta Hydroxybutyric Acid (OHb) ELISA
猪低密度脂蛋白(LDL)检测盒Pig Low Density Lipoprotein (LDL) ELISA
猪双糖链蛋白聚糖(BGN)检测盒Pig Biglycan (BGN) ELISA
猪甲胎蛋白(αFP)检测盒Pig Alpha-Fetoprotein (aFP) ELISA
猪AMP激活蛋白激酶α1(PRKAα1)检测盒Pig Protein Kinase, AMP Activated Alpha 1 (PRKAa1) ELISA
猪衰老关键蛋白2(FBLN2)检测盒Pig Fibulin 2 (FBLN2) ELISA
猪*酶(CAT)检测盒Pig Catalase (CAT) ELISA
猪白介素6(IL6)检测盒Pig Interleukin 6 (IL6) ELISA
猪α1-酸性糖蛋白(α1AGP)检测盒Pig Alpha-1-Acid Glycoprotein (a1AGP) ELISA
人信号素3A(SEMA3A)测定盒Human Semaphorin 3A (SEMA3A) ELISA
