ELISA是以免疫学反应为基础，将抗原、抗体体的特异性反应与酶对底物的高效催化作用相结合起来的一种敏感性很高的试验技术。
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Pig Angiopoietin Like Protein 4 (ANGPTL4) ELISA
在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次
ELISA results using S-OIV A neuraminidase antibody at 1 μg/ml to probe the immunogenic and the corresponding seasonal influenza A neuraminidase peptides at 50, 10, 2, and 0 ng/ml. Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[3] or West Nile Virus). It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.[4] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs. The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400-fold and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme. Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, for example, is established, and a sample that contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test.
猪核因子κB受体激活因子配基(RANκL)检测盒Pig Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ELISA
猪蛋白酶激活亚基3(PSME3)检测盒Pig Proteasome Activator Subunit 3 (PSME3) ELISA
猪硬骨素(SOST)检测盒Pig Sclerostin (SOST) ELISA
人三角形四肽重复干扰素诱导蛋白1(IFIT1)测定盒Human Interferon Induced Protein With Tetratricopeptide Repeats 1 (IFIT1) ELISA
人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)测定盒Human Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) ELISA
人蛋白C抑制因子(PCI)测定盒Human Protein C Inhibitor (PCI) ELISA
猪睾酮(Testosterone)检测盒Pig Testosterone
猪组织蛋白酶S(CTSS)检测盒Pig Cathepsin S (CTSS) ELISA
猪亲环素A(CYPA)检测盒Pig Cyclophilin A (CYPA) ELISA
人纤维蛋白肽A(FPA)测定盒Human Fibrinopeptide A (FPA) ELISA
人硫氧化还原蛋白(Trx)测定盒Human Thioredoxin (Trx) ELISA
人干扰素诱导蛋白41(IFI41)测定盒Human Interferon Induced Protein 41, 30kDa (IFI41) ELISA
