人脂肪细胞脂肪酸结合蛋白(aFABP)elisa检测试剂盒，英文名：Human adipocyte fatty-acid binding protein,AFABP ELISA Kit
产品简单说明：脂肪细胞脂肪酸结合蛋白(adipocyte fatty-acid binding protein,AFABP)属于细胞内脂肪酸结合蛋白(AFBP)家族，分子量15kDa。脂肪酸结合蛋白(AFBP)具有在腔内结合脂肪酸及相关复合物(胆汁酸或类维生素A)的能力。AFABP在脂肪细胞中分化依赖性表达，是调节脂肪细胞生物功能的重要基因。
更多人ELISA试剂盒产品！
ELISA的比色测定由酶标仪进行。有的同行可能会认为，既然由酶标仪进行，那么此时便可以万事大吉了，其实不然，因为当代较为先进的酶标仪，均有较多的功能，使用不当，会得到令人难以理解的人脂肪细胞脂肪酸结合蛋白(aFABP)elisa检测试剂盒实验结果。如使用酶标仪比色测定后，有许多的阴性测定孑L的吸光度值为负数；或测定假阳性率大大增加等等。这主要是没有正确地理解和使用酶标仪所致。
此外，在加入底物开始显色反应前，是先检查一下底物溶液的有效性，即可将A和B两种液各加1滴于清洁的空板孔或eppendorf管中，观察是否有显色出现，如有，则说明底物已变质。以OPD为底物，配好后应为五色，否则就不能使用。以TMB为底物，整个显色反应过程无需避光，而以OPD为底物，则需避光进行。关于TMB和OPD的显色反应特点及注意点可参考前面有关章节内容。显色反应完成后，加入酸终止反应，振荡混匀后即可进行下面的比色测定或肉眼判断结果。
Y136521 人食欲素/阿立新B(OX-B)ELISA试剂盒 Human Orexin B,OX-B ELISA Kit 96T/48T
Y136522 人神经元凋亡抑制蛋白(NAIP)ELISA试剂盒 Human neuronal apoptosis inhibitory protein,NAIP ELISA Kit 96T/48T
Y136523 人多巴胺D2受体(D2R)ELISA试剂盒 Human dopamine D2 receptor,D2R ELISA Kit 96T/48T
Y136524 人神经调节蛋白1(NRG-1)ELISA试剂盒 human Neuregulin 1,NRG-1 ELISA Kit 96T/48T
Y136525 人脑源性神经营养因子(BDNF)ELISA试剂盒 Human Brain derived neurotrophic facor,BDNF ELISA Kit 96T/48T
Y136526 人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA试剂盒 Human E-Cadherin,E-Cad ELISA Kit 96T/48T
Y136527 人二级淋巴组织趋化因子(SLC/CCL21)ELISA试剂盒 Human secondary lymphoid-tissue chemokine,SLC ELISA Kit 96T/48T
Y136528 人XC趋化因子受体1(XCR1)ELISA试剂盒 Human XC-chemokine receptor 1,XCR1 ELISA Kit 96T/48T
Y136529 人CXC趋化因子受体1(CXCR1)ELISA试剂盒 Human CXC-chemokine receptor 1,CXCR1 ELISA Kit 96T/48T
Y136530 人睫状神经营养因子(CNTF)ELISA试剂盒 Human Ciliary Neurotrophic Factor,CNTF ELISA Kit 96T/48T
Y136531 人内分泌腺来源的血管内皮生长因子(EG-VEGF)ELISA试剂盒 Human Endocrine gland vascular endothelial growth factor,EG-VEGF ELISA Kit 96T/48T
Y136532 人嗜酸粒细胞趋化蛋白Eotaxin 1(Eotaxin 1/CCL11)ELISA试剂盒 Human Eotaxin 1 ELISA Kit 96T/48T
Y136533 人凋亡相关因子(FAS/CD95)ELISA试剂盒 Human Factor-related Apoptosis,FAS ELISA Kit 96T/48T
Y136534 人凋亡相关因子配体(FASL)ELISA试剂盒 Human Factor-related Apoptosis ligand,FASL ELISA Kit 96T/48T
Y136535 人酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒 Human acidic fibroblast growth factor 1,aFGF-1 ELISA Kit 96T/48T
Y136536 人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒 Human basic fibroblast growth factor 4,bFGF-4 ELISA Kit 96T/48T
Y136537 人碱性成纤维细胞生长因子6(bFGF-6)ELISA试剂盒 Human basic fibroblast growth factor 6,bFGF-6 ELISA Kit 96T/48T
Y136538 人粒细胞集落刺激因子(G-CSF)ELISA试剂盒 Human Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit 96T/48T
Y136539 人胶质细胞系来源的神经营养因子(GDNF)ELISA试剂盒 Human glial cell line-derived neurotrophic factor,GDNF ELISA Kit 96T/48T
Y136540 人粒细胞巨噬细胞集落刺激因子(GM-CSF)ELISA试剂盒 Human Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit 96T/48T
Y136541 人胰岛素样生长因子结合蛋白1(IGFBP-1)ELISA试剂盒 Human insulin-like growth factors binding protein 1,IGFBP-1 ELISA Kit 96T/48T
Y136542 人胰岛素样生长因子结合蛋白2(IGFBP2)ELISA试剂盒 Human insulin-like growth factors binding protein 2,IGFBP-2 ELISA Kit 96T/48T
Y136543 人胰岛素样生长因子结合蛋白3(IGFBP-3)ELISA试剂盒 Human insulin-like growth factors binding protein 3,IGFBP-3 ELISA Kit 96T/48T
Y136544 人单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)ELISA试剂盒 Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA Kit 96T/48T
Y136545 人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒 Human monocyte chemotactic protein 2,MCP-2 ELISA Kit 96T/48T
Y136546 人单核细胞趋化蛋白3(MCP-3/CCL7)ELISA试剂盒 Human monocyte chemotactic protein 3,MCP-3 ELISA Kit 96T/48T
Y136547 人巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒 Human Macrophage Inflammatory Protein-1α,MIP-1α ELISA Kit 96T/48T
Y136548 人巨噬细胞炎性蛋白1β(MIP-1β/CCL4)ELISA试剂盒 Human Macrophage Inflammatory Protein-1β,MIP-1β ELISA Kit 96T/48T
Y136549 人巨噬细胞炎性蛋白3α(MIP-3α/CCL20)ELISA试剂盒 Human Macrophage Inflammatory Protein-3α,MIP-3α ELISA Kit 96T/48T
Y136550 人巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA试剂盒 Human Macrophage Inflammatory Protein-3β,MIP-3β ELISA Kit 96T/48T
Y136551 人的神经生长因子(NGF)ELISA试剂盒 Human Nerve growth factor,NGF ELISA Kit 96T/48T
Y136552 人神经营养因子3(NT-3)ELISA试剂盒 人神经营养因子3(NT-3)ELISA Kit 96T/48T
Y136553 人神经营养因子4(NT-4)ELISA试剂盒 Human Neurotrophin 4,NT-4 ELISA Kit 96T/48T
Y136554 人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒 Human regulated on activation in normal T-cell expressed and secreted,RANTES ELISA Kit 96T/48T
Y136555 人干细胞因子受体(SCFR)ELISA试剂盒 Human Stem Cell Factor Receptor,SCFR ELISA Kit 96T/48T
Y136556 人基质细胞衍生因子1β(SDF-1β/CXCL12)ELISA试剂盒 Human Stromal cell derived factor 1β,SDF-1β ELISA Kit 96T/48T
Y136557 人肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒 Human Tumor necrosis factor soluble receptor Ⅰ,TNFsR-ⅠELISA Kit 96T/48T
Y136558 人肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)ELISA试剂盒 Human Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA Kit 96T/48T
Y136559 人肿瘤坏死因子相关凋亡诱导配体1(TRAIL-R1)ELISA试剂盒 Human tumor necrosis factor-related apoptosis-inducing ligand 1,TRAIL-R1 ELISA Kit 96T/48T
Y136560 人肿瘤坏死因子相关凋亡诱导配体3(TRAIL-R3)ELISA试剂盒 Human tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit 96T/48T
Y136561 人肿瘤坏死因子相关凋亡诱导配体4(TRAIL-R4)ELISA试剂盒 Human tumor necrosis factor-related apoptosis-inducing ligand 4,TRAIL-R4 ELISA Kit 96T/48T
Y136562 人可溶性肿瘤坏死因子相关凋亡诱导配体(sTRAIL)ELISA试剂盒 Human soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL ELISA Kit 96T/48T
Y136563 人血管内皮细胞生长因子C(VEGF-C)ELISA试剂盒 Human Vascular Endothelial cell Growth Factor C,VEGF-C ELISA Kit 96T/48T
Y136564 人血管内皮细胞生长因子D(VEGF-D)ELISA试剂盒 Human Vascular Endothelial cell Growth Factor D,VEGF-D ELISA Kit 96T/48T
Y136565 人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒 Human Vascuoar endothelial cell growth factor receptor 1,VEGFR-1/Flt1 ELISA Kit 96T/48T
Y136566 人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒 Human Vascuoar endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA Kit 96T/48T
Y136567 人B细胞活化因子受体(BAFF-R)ELISA试剂盒 Human B cell activation factorr from the tumor necrosis factor family receptor,BAFF-R ELISA Kit 96T/48T
Y136568 人结缔组织生长因子(CTGF)ELISA试剂盒 Human connective tissue growth factor,CTGF ELISA Kit 96T/48T
Y136569 人巨噬细胞炎性蛋白5(MIP-5)ELISA试剂盒 Human macrophage inflammatory protein 5,MIP-5 ELISA Kit 人脂肪细胞脂肪酸结合蛋白(aFABP)elisa检测试剂盒 96T/48T
Y136570 人干扰素诱导蛋白10(IP-10/CXCL10)ELISA试剂盒 Human interferon-inducible protein 10,IP-10 ELISA Kit 96T/48T
在目前的以HRP作为标记酶的商品市场ELISA试剂盒中，如以TMB为底物，则提供的底物为A和B两瓶应用液；如以OPD为底物，则试剂盒提供OPD片剂或粉剂，临用前配制。一般商品人脂肪细胞脂肪酸结合蛋白(aFABP)elisa检测试剂盒显色反应条件为37℃或室温反应15～30min。从理论上说，37℃30min才可以使HRP的底物催化反应*，尽管在zui初的10min内，绝大部分催化反应即可完成。因此，为使弱阳性样本孔能有充分的显色，建议在37℃下反应25～30min后，终止反应比色测定。
