Osteocalcin/FITC说明书

                  Anti-Osteocalcin/FITC说明书

Catalog Number :Rs-4917R-FITC

Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ） Background:

Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. Function : Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.Subcellular Location : Secreted.Post-translational modifications : Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Similarity : Belongs to the osteocalcin/matrix Gla protein family.

Contains 1 Gla （gamma-carboxy-glutamate） domain.

Specificity : Anti-Osteocalcin/FITC is a mouse Monoclonall antibody             

specific for Osteocalcin/FITCof Human, Rb

• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:  11kDa

Application :  

• Flow-cyt=1:50-200  IF=1:50-200
• Emission spectrum:490-510nm
• not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.