Anti-AQP3抗体,水通道蛋白3抗体说明书 >>>>>>点击查看
Rabbit Anti-AQP3 antibody
Catalog Number : BYK-1253R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 （major intrinsic protein 26kD）, and Aquaporin-1 （AQP1, purified from red cells） also called CHIP-28 （channel forming integral protein, 28kD; 268aa; gene locus 7p14） has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation （CAT-FR or Shriveled） is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation （LOP） is an amino acid substitution that inhibits targeting of MIP to the cell membrane.
AQP3 （GLIP, Glycerol-transporting integral protein; 28a; 31.4kD） has a wide tissue distribution. AQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It is also slightly permeable to urea and glycerol. AQP3 may play an important role in gastrointestinal tract water transport and in glycerol metabolism. In the kidney it is predominantly expressed in the inner medulla. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
Anti-AQP3抗体,水通道蛋白3抗体说明书Specificity :
– Anti-AQP3 is a rabbit polyclonal antibody unconjugated
– specific for AQP3 of hu, mo, rat
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 32kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– IHC-P=1:100-500 IHC-F=1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Anti-AQP3抗体,水通道蛋白3抗体说明书Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
