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当前位置:上海纪宁酶联科技有限公司>技术文章>ELSIA试剂盒监测伤*鞭毛抗原H抗体相关报告

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技术文章

ELSIA试剂盒监测伤*鞭毛抗原H抗体相关报告

阅读:414发布时间:2014-4-11

ELSIA试剂盒监测沙门氏杆菌的h抗原有两种,称为第1相和第2相。第1相特异性高,又称特异相,用a、b、c等表示,第2相特异性低,为数种沙门氏杆菌所共有,也称非特异相,用1、2、3等表示。具有第1相和第2相h抗原的细菌称为双相菌,仅有一相者称单相菌。每一组沙门氏杆菌根据h抗原不同,可进一步分种或型。h抗原刺激机体主要产生lgG抗体。
英文名称  Anti-Salmonella enteritidis H 
中文名称  伤*鞭毛抗原H抗体 
别    名  Ribonuclease H; RNase H; RNH_SALEP. 
浓    度  1mg/1ml 
规 格  0.2ml/200μg  1ml/1mg 
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Mouse, Salnellaenteritidis  
产品类型  一抗    
研究领域  免疫学 细菌及病毒  
蛋白分子量  predicted molecular weight: 17kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from Sa Salmonella enteritidis H 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
ELSIA试剂盒监测伤*鞭毛抗原H抗体应用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 
(石蜡切片需做抗原修复) 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram-negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors. The commonest Salmonella serotype associated with food borne infections in humans is Salmonella enteriditis and in particular phage type 4 (PT4). Salmonella Enteriditis bacteria may be found in the intestinal tracts of livestock, poultry, dogs, cats and other warm-blooded animals. This strain is only one of about 2,000 kinds of Salmonella bacteria; it is often associated with poultry and eggs.
Function : Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (By similarity).
Subunit : Monomer (By similarity). 
Subcellular Location : Cytoplasm (Potential).
Similarity : Belongs to the RNase H family. 
Contains 1 RNase H domain. 
Database links : UniProtKB/Swiss-Prot: B5R449.1
 

 


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