大鼠雌激素受体elisa试剂盒原理注意事项（英文版）

The experiment principle
This kit using double antibody method to determine the specimen cuhk rat estrogen receptor （ER） level. Rats with purified antibody of estrogen receptor （ER） package is MPP, made from solid phase antibody, the bags in the sheet resistance of the microporous, in turn, to join the estrogen receptor （ER）, again with the HRP labeled estrogen receptor （ER） antibodies, antibody antigen - enzyme mark antibody complex formation, after thoroughly wash with TMB chromogenic substrates. TMB under the catalysis of HRP enzyme into blue, and under the action of acid into the yellow. The depth of the color and the estrogen receptor （ER） in the samples were positively correlated. Enzyme standard instrument under 450 nm wavelength determination of absorbance （OD value）, cuhk rat by standard curve calculation sample concentration of estrogen receptor （ER）.
Matters needing attention
1. The kit should be at room temperature out of the cold storage environment balance can be used only for 15 to 30 minutes, and enzyme standard package is board if not used up after opening, slats should be kept in sealed bag.
2. There may be crystallization precipitation concentrated washing liquid, when diluted in water bath heating to dissolve and washing does not affect the results.
Step Step

3. The sample add washer should be used, and regularly check its accuracy, to avoid the test errors. A best sample time control within 5 minutes, such as the number of specimens, it is recommended to use volley and sample.
4 Please do test at the same time standard curve at a time, preferably do complex hole. Such as excessive amounts of material under test in the specimen （the sample OD value is greater than standard holes on the first hole of the OD value）, please use the sample diluent dilution multiples （n times） must be after the measurement, calculation, please the last times the total dilution multiples （* n * 5）.
5 Sealing plate membrane only for one-time use, in order to avoid cross contamination.
6. The substrate please avoid light preservation.
7. In strict accordance with the manual operation, the test result determination must take enzyme mark meter readings shall prevail.
8. All samples, washing liquid and all kinds of waste should be handled according to the infection.
9. The different batch number components shall not be mixed reagent.
10. If there are different with English manual, the English instruction manual shall prevail.