【文献标题】p-Hydroxybenzoic acid alleviates inflammatory responses and intestinal mucosal damage in DSS-induced colitis by activating ERβ signaling

【作者】Xiaotian Xu , Ailin Luo , Xi Lu , et.al

【作者单位】桂林医学院（Guilin Medical University）

【文献中引用产品】

小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

小鼠白介素6(IL-6)ELISA试剂盒

小鼠白介素1β (IL-1β)ELISA试剂盒

【关键词】Ulcerative colitis ,P-hydroxybenzoic acid ,Inflammatory response ,Intestinal mucosal damage ,Estrogen receptor β 

【DOI】10.1016/j.jff.2021.104835

【影响因子(IF)】4.45

【出版期刊】《Journal of Functional Foods》

【产品原文引用】

DSS (molecular weight: 36–50 kDa) was purchased from MP Biomedicals (Solon, USA). HD (C7H6O2, molecular weight: 122.12, purity, ≥ 98%) and HA (C7H6O3, molecular weight: 138.12, purity, ≥ 98%) were purchased from Yuanye Biology (Shanghai, China). Mesalazine sustained release granules were purchased from Ipsen Pharma (Houdan, France). 4-[2-Phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]-pyrimidin-3-yl] phenol (PHTPP)-selective ERβ antagonist (C20H11F6N3O, molecular weight: 423.31, purity, >99%) was acquired from Tocris Bioscience (Missouri, USA). Recombinant tumor necrosis factor-alpha (TNF-α) was provided by R&D Systems, Inc. (California, USA). TNF-α, interleukin 6 (IL-6) and interleukin-1 beta (IL-1β) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Hengyuan Biotech Co., Ltd. (Shanghai, China). An MPO activity assay kit was obtained from Nanjing Jiancheng of the Bioengineering Institute (Nanjing, China). HiScript RT Super Mix and Ace Q-PCR SYBR Green Master Mix were obtained from Vazyme Co., Ltd. (Nanjing, China). Rabbit monoclonal antibodies against E-cadherin (#BS1098) and β-actin (#BS6007 M) were provided by Bioworld (Minnesota, USA). The rabbit monoclonal antibody occludin (#27260-1-AP) was purchased from Proteintech (Wuhan, China). The rabbit monoclonal antibody ERβ (#bs- 0116R) was purchased from Bioss Antibodies (Beijing, China). All chemical reagents used in this study were analytical grade or chromatographic grade. 

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