【文献标题】MiRNA-34c-5p protects against cerebral ischemia/reperfusion injury:involvement of anti-apoptotic and anti-inflammatory activities

【作者】Yaoran Tu1 & Yong Hu

【作者单位】南昌大学（Nanchang University）

【文献中引用产品】

β-actin抗体

抗兔二抗—抗体

【关键词】miR-34c-5p . Cerebral ischemia . Inflammation . Apoptosis

【DOI】10.1007/s11011-021-00724-5

【影响因子(IF)】3.58

【出版期刊】《Metabolic Brain Disease》

【产品原文引用】

Western blotting was performed as previously described (Sayre et al. 2010). Protein concentrations were quantified using the BCA Protein Assay Kit. After denaturing in boiling water for 10 min, protein samples were separated using SDSPAGE gel (10%), followed by transferring to PVDF membrane. The membrane was incubated with anti-P65 antibody (1:500, Youliante, Wuhan, China), Bcl-2 antibody (1:1000, Youliante, Wuhan, China), Bax antibody (1:1000, Youliante, Wuhan, China), COX-2 antibody (1:500, Youliante, Wuhan, China), iNOS antibody (1:500, Youliante, Wuhan, China), NCOA1 antibody (1:500, Youliante, Wuhan, China) and β-actin antibody (1:1000, Hengyuan, Shanghai, China) overnight. Then anti-rabbit secondary antibody (1:1000, Hengyuan, Shanghai, China) was added for incubation of another 1 h.

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