【文献标题】Short-chain aldehydes increase aluminum retention and sensitivity by enhancing cell wall polysaccharide contents and pectin demethylation in wheat seedlings

【作者】Xin Liang, Yiqun Ou, Hongcheng Zhao,et.al

【作者单位】浙江大学（Zhejiang University）

【文献中引用产品】

果胶甲酯酶（PME）ELISA试剂盒 活性

【关键词】Al toxicity ,Cell wall ,Pectin methylation ,Short-chain aldehyde ,Al accumulation ,Wheat 

【DOI】10.1016/j.jhazmat.2022.128743

【影响因子(IF)】10.58

【出版期刊】《Journal of Hazardous Materials》

【产品原文引用】

2.12. Detection of PME activity 

Pectin methylesterase activity was measured using an ELISA kit (Shanghai Hengyuan Biological Technology Co., Ltd). Approximately 

0.1 g fresh weight of root tips (0–10 mm) was homogenized in 0.9 mL of PBS (50 mM, pH 7.0), and the extracts were centrifuged at 12,000g at 4 ℃ for 20 min. The crude enzyme extracts were used to determine the activities of pectin methylesterase by following the manufacturer’s instructions. 

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