【文献标题】Dehydroepiandrosterone alleviates intestinal inflammatory damage via GPR30-mediated Nrf2 activation and NLRP3 inflammasome inhibition in colitis mice

【作者】Ji Cao , Miaomiao Lu , Weiyuan Yan , et.al

【作者单位】南京农业大学（Nanjing Agricultural University）

【文献中引用产品】

人肿瘤坏死因子α(TNF-α)ELISA试剂盒

人白细胞介素1β(IL-1β)ELISA试剂盒

人雌二醇(E2)ELISA试剂盒

【关键词】Dehydroepiandrosterone ,GPR30 ,Nrf2 ,NLRP3 inflammasome ,Colitis

【DOI】10.1016/j.freeradbiomed.2021.06.025

【影响因子(IF)】7.37

【出版期刊】《Free Radical Biology and Medicine》

【产品原文引用】

Sigma (St Louis, MO, USA) provided the DHEA, dimethyl sulfoxide (DMSO) and LPS (Escherichia coli 055:B5). The Opti-MEM medium and fetal bovine serum (FBS) were obtained from Gibco (Erie, NY, USA). The dulbecco's modified Eagle's medium (DMEM) and trypsin-EDTA were all obtained from Biological Industries (Kibbutz Beit-Haemek, Israel). Corning (NY, USA) provided the cell culture dishes.

TRIZOL Reagent Kits, SYBR Green Master Mix and reverse transcriptase kit were all purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). Jiancheng Bioengineering Institute of Nanjing (Nanjing, China) provided the Nitric Oxide (NO), malondialdehyde (MDA) and total superoxide dismutase (SOD) detection kits. Shanghai hengyuan biological technology Co., Ltd. (Shanghai, China) provided the

TNF-α, IL-1β and Estradiol (E2) ELISA kits. Beyotime Biotechnology Institute (Shanghai, China) provided the glutathione peroxidase, nitric Oxide (NO), protein contents assay kits and N-Acetyl-L-cysteine (NAC). MCE (St. Louis, MO, USA) provided the inhibitors including G1, G15, SB203580, U0126, BAY11-7082, GKT137831, TAK-242, 3-MA, Fulvestrant, Flutamide, ML385 and RTA-408.

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