【文献标题】MUC5B regulates the airway inflammation induced by atmospheric PM2.5 in rats and A549 cells

【作者】Liting Zhou , Hongbo Liu , Ruxuan Zhang , et.al

【作者单位】吉林大学（Jilin University）

【文献中引用产品】

大鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

大鼠白介素1β (IL-1β)ELISA试剂盒

大鼠粘蛋白粘液素5B（MUC5B）ELISA试剂盒

【关键词】PM2.5 ,MUC5B ,Airway inflammation ,Mucin secretion 

【DOI】doi.org/10.1016/j.ecoenv.2021.112448

【影响因子(IF)】8.2

【出版期刊】《Ecotoxicology and Environmental Safety》

【产品原文引用】

2.8. Enzyme-linked immunosorbent assay (ELISA) analysis 

ELISA (Hengyuan, Shanghai, China) was used to detect the levels of inflammatory factors (TNF-α and IL-1β) in bronchoalveolar lavage fluid 

and serum of rats and in cell culture, MUC5B in trachea and lung of rats and in cell culture were detected with ELISA (Hengyuan, Shanghai, 

China), according to the manufacturer’s instructions. The levels of TNF- α, IL-1β and MUC5B were quantified with the Epoch Microplate Spectrophotometer (Bio-Rad Laboratories, Hercules, California, USA), and determined by comparing the optical density of the specimens to the standard curve at 450 nm. 

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