【文献标题】Enantioselective bioaccumulation and detoxification mechanisms of earthworms (Eisenia fetida) exposed to mandipropamid

【作者】Kuan Fang , Lingxi Han , Yalei Liu ,et.al

【作者单位】中国农业科学院（Chinese Academy of Agricultural Sciences）

【文献中引用产品】

蚯蚓细胞色素P450(CYP450)ELISA试剂盒 酶活

蚯蚓羧酸酯酶(CarE)ELISA试剂盒 酶活

蚯蚓（GST）ELISA试剂盒 酶活

【关键词】Enantioselectivity,Mandipropamid enantiomers,Detoxification enzymes,Transcriptome sequencing,Detoxification mechanisms

【DOI】doi.org/10.1016/j.scitotenv.2021.149051

【影响因子(IF)】10.5

【出版期刊】《Science of the Total Environment》

【产品原文引用】

2.4. Determination of detoxification enzyme activities and the secondgeneration integrated biomarker response index (IBRv2)

One earthworm was randomly selected from each beaker, weighed,and homogenized with a 50 mM phosphate buffer (pH 7.8; 1:10, W/V). The homogenized mixture was then centrifuged in a cryogenic centrifuge at 9000 rpm for 10 min, and the collected supernatant was analyzed to determine the activities of CYP450, CarE, and GST using a kit provided by ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The absorbance (optical density value) was determined at a wavelength of 450 nm using a microplate reader, and the activities of CYP450, CarE, and GST in the sample were calculated from the standard curve.

完整版PDF文献请咨询在线客服或者联系我司业务员！

更多公司福利请关注“恒远生物"微信公众号！