【文献标题】Therapeutic effect of human amniotic epithelial cells in murine models of Hashimoto’s thyroiditis and Systemic lupus erythematosus

【作者】BING TAN，PENGJIE YANG，JINYING LI，et.al

【作者单位】浙江大学（Zhejiang University）

【文献中引用产品】

小鼠白介素17A（IL-17A）ELISA试剂盒

小鼠γ干扰素（IFN-γ）ELISA试剂盒

小鼠白介素4（IL-4）ELISA试剂盒

小鼠白介素10（IL-10）ELISA试剂盒

小鼠转化生长因子β（TGF-β） ELISA试剂盒

【关键词】cell therapy, experimental autoimmune thyroiditis, human amniotic epithelial cells, Hashimoto’s thyroiditis，immunomodulatory, Systemic lupus erythematosus

【DOI】doi.org/10.1016/j.jcyt.2018.04.001

【影响因子(IF)】3.84

【出版期刊】《International Society》

【产品原文引用】

Serological assays

Sera cytokine levels of IL-17A, IFN-γ, IL-4, IL-10 and TGF-β of EAT and SLE mice were measured by ELISA. All ELISA kits were from Hengyuan Biological Technologies (Shanghai, China). Specific experimental steps were performed according to the manufacturer’s instructions.

Cytokine production in vitro

Splenocytes of EAT mice at the peak of disease (35 day) were stimulated with pTg in the presence or absence of hAECs. Cell-free culture supernatants were collected after 48 h by centrifugation. Cytokine levels of IL-17A, IFN-γ,IL-4, IL-10 and TGF-β were measured by ELISA(Hengyuan BiologicalTechnologies, Shanghai, China).

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