【文献标题】 The distinct role of CD73 in the progression of pancreatic cancer

【作者】Liangjing Zhou1，Shengnan Jia1，Yan Chen，et.al

【作者单位】浙江大学（Zhejiang University）

【文献中引用产品】

人肿瘤坏死因子α（TNF-α）ELISA试剂盒

【关键词】Pancreatic cancer，Progression，CD73，TNFR2，miR-30a-5p

【DOI】doi.org/10.1007/s00109-018-01742-0

【影响因子(IF)】4.14

【出版期刊】 《Journal of Molecular Medicine》

【产品原文引用】 

Detection of TNF-α levels in supernatant via ELISA

Supernatant was investigated for levels of TNF-α protein using commercial TNF-α ELISAs (Hengyuan Biotechnology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions.

Western blot

Cells were harvested, and total protein lysates were resolved using 10% sodium dodecyl sulfate–polyacrylamide gels,electroblotted onto a polyvinylidene fluoride membrane,blocked by 5% skim milk, and probed with primary antibodies listed in Table S1. The membrane was then incubated with the secondary antibody and visualized by enhanced chemiluminescence using Kodak X-Omat LS film (Eastman Kodak,Rochester, USA).

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