【文献标题】Effects of dietary protein to energy ratios on growth, body composition and digestive enzyme activities in Chinese mitten-handed crab, Eriocheir sinensis

【作者】Yanyan Cui，Qianqian Ma，Samwel Mchele Limbu，et.al

【作者单位】华东师范大学（East China Normal University）

【文献中引用产品】

螃蟹蛋白酶（Protease）ELSIA试剂盒

螃蟹脂肪酶（Lipase）ELISA试剂盒

【关键词】Eriocheir sinensis, dietary protein to energy ratio, lipid, growth, body composition digestive enzyme

【DOI】doi:10.1111/are.13061

【影响因子(IF)】1.6

【出版期刊】《Aquaculture Research》

【产品原文引用】

To measure the digestive enzymes activities, the hepatopancreas samples were weighed and homogenized in 10 volumes (v/w) of pre-chilled phosphate buffer solution (pH 7.3). The homogenates were then centrifuged at 2500 r min1 for 30 min and the supernatants were collected. Amylase activity was determined by using the iodinestarch colorimetric method by utilizing assay kits from Nanjing Jiancheng Bioengineering Institute (Cat. No. C016, Nanjing, China). Total protease and lipase activities were measured by using enzyme-linked immune sorbent assay (ELISA) kit purchased from Shanghai Hengyuan Biotechnology. One unit of amylase activity (U mg1 protein)was defined as one mg substrate hydrolyzing 10 mg starch in 30 min at 37°C. The protein concentration of supernatants was measured as described by Bradford (1976).

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