【文献标题】LncRNA MEG3 inhibits the infammatory response of ankylosing spondylitis by targeting miR‑146a

【作者】Yehong Li，Shanshan Zhang，Cunxin Zhang，et.al

【作者单位】济宁市第一人民医院

【文献中引用产品】

人白细胞介素-1β（IL-1β）ELISA试剂盒

人白细胞介素-6（IL-6）ELISA试剂盒

人肿瘤坏死因子-α(TNF-α)ELISA试剂盒

【关键词】Ankylosing spondylitis，MEG3，miR-146a，Infammatory response，Cytokines

【DOI】doi.org/10.1007/s11010-019-03681-x

【影响因子(IF)】5.10

【出版期刊】《Molecular and Cellular Biochemistry》

【产品原文引用】

Enzyme‑linked immunosorbent assay (ELISA)

The levels of infammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were detected by ELISA kit (Hengyuan, Shanghai, China). In short, the samples to be tested and the standard samples were added to the corresponding enzyme plate and cultured at 37 ℃ for 1 h. After washing with PBS, the chromogenic antibody was added to the enzyme plates and continued incubation for 30 min. Finally, the termination solution was added to terminate the reaction. OD value at 450 nm was measured by a microplate reader (Thermo Fisher Scientifc, Rockford, IL, USA).

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