人胚肝干细胞培养基说明书

OriCell Human Embryonic Stem Cell Growth Medium

Catalog No. HUXES-90011

Product Description:

Human Embryonic Stem Cell Growth Medium consists of optimized Human Embryonic Stem Cell
Basal Medium, pre-selected Fetal Bovine Serum and supplements. This product has been developed for
the optimal expansion and maintenance of Human Embryonic Stem Cells （Cat. No. HUXES-01001）. It
maintains the undifferentiated state and retains the pluripotential phenotype of Human Embryonic Stem
Cells.
The product is intended for laboratory research use only, not for drug, house hold, or other uses.

Kit Components:

Human Embryonic Stem Cell Basal Medium 385 mL
（Cat. No. HUXES-03011-385）
Serum Replacement 60 mL
（Cat. No. SRN-19001-60）
Human Embryonic Stem Cell-Qualified Fetal Bovine Serum 40 mL
（Cat. No. HUXES -05001-40）
Penicillin-Streptomycin 5 mL
Glutamine 5 mL
Non-essential Amino Acid 5 mL
bFGF （0.5 mg/mL） 100 μL
2-Mercaptoethanol 3.5 μL

Instructions for Use:

1. Prior to use, thaw Human Embryonic Stem Cell-Qualified Fetal Bovine Serum and Serum
Replacement under refrigeration （2 to 8°C） over night or until compley thawed. Gently swirl the
bottles to ensure homogeneity. Human Embryonic Stem Cell-Qualified Fetal Bovine Serum has been
heat-inactivated and is ready to use after thawing.
Note: The thawed serum may contain some flocculent precipitates. The presence of these
substances in serum does not alter the performance characteristics of the product. It is not
recommended to filter the serum to remove these precipitates. Doing so may result in the loss of
some serum nutrients.
2. About 30 minutes prior to use, thaw Penicillin-Streptomycin solution, Glutamine solution and
Non-essential Amino Acid solution at room temperature. Gently swirl the vials to ensure
homogeneity.
3. About 10 minutes prior to use, thaw bFGF and 2-Mercaptoethanol at room temperature.