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ROS Brite APF

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产品型号AAT-16050

品       牌

厂商性质代理商

所  在  地武汉市

更新时间:2021-10-13 15:56:35浏览次数:17次

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中文名称
ROS Brite APF
英文名字
ROS Brite APF
供应商
AAT Bioquest
产品货号
AAT-16050
  • 产品新闻

  • 背景资料

  • Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen。 Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides。 ROS is highly reactive due to the presence of unpaired valence shell electrons。 ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis。 However, during times of environmental stress (e。g。, UV or heat exposure), ROS levels can increase dramatically。 This may result in significant damage to cell structures。 Cumulatively, this is known as oxidative stress。 ROS are also generated by exogenous sources such as ionizing radiation。 Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids。 Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders。 ROS Brite APF is a fluorogenic probe to measure hydroxyly radical in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry。 The cell-permeant ROS Brite APF reagent is nonfluorescent and produces bright green fluorescence upon reaction with hydroxyl radical。 The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry。 In the presence of peroxidase, APF also reacts with hydrogen peroxide。 APF has good selectivity to hydroxyl radical compared to other ROS。 APF and HPF show relatively high resistance to light-induced oxidation。 APF and HPF are nonfluorescent until they react with the hydroxyl radical or peroxynitrite anion。 APF will also react with the hypochlorite anion。

  • 产品描述

  • ROS Brite APF产品的光谱学相关参数为Ex(nm)=492/ Em(nm)515;其对应分子量大小为423。42。

  • 产品形式

  • 提供的ROS Brite APF产品为固体形式,我们建议将其溶解到DMSO溶液中使用或保存。

  • 保存建议

  • 对于ROS Brite APF产品,我们推荐使用常温进行运输。在您收到货物后,我们建议在低于-15℃温度下冷冻保存,要求储存环境干燥并避免光照。产品有效期为12个月。

  • 其他

  • 美国AAT Bioquest Inc。(前身是ABD Bioquest,Inc。)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助 的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。作为AAT Bioquest Inc。的中国区域代理,艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析 的产品,包括显色,荧光和发光技术等全系列解决方案。


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