Recombinant Protein A/G fusion protein joins IgG binding domains of both Protein A and Protein G. Protein A/G includes four Fc binding domains from Protein A and two from Protein G， yielding a final mass of 50.4 kDa. The binding dependency to pH of Protein A/G lower than Protein A， but has the additive properties of Protein A and G together. Protein A/G binds to all subclasses of human IgG， making it helpful for purifying polyclonal or monoclonal IgG antibodies whose subclasses have not been identifieed. Protein A/G binds to IgA， IgE， IgM and IgD. Protein A/G binds to all subclasses of mouse IgG excluding mouse IgA， IgM or serum albumin. This permits Protein A/G to be used in purification and detection of mouse monoclonal IgG antibodies， with no interference from IgA， IgM and serum albumin. Mouse monoclonal antibodies normally have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G. Protein A/G also has been used for purification of macaque IgG.
Lyophilized white Powder containing no additives.
2 years at -20℃. After reconstitution， aliquot and store at -20℃. Avoid repeated freeze/thaw cycles.