艾美捷cAMP直接ELISA试剂盒解决方案来啦

Cyclic AMP (cAMP) is one of the most important “second messengers" involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

Direct cAMP ELISA kit：

Most sensitive (0.006 pmol/ml) colorimetric kit for intracellular cAMP quantification.

Replaces Prod. #: ADI-900-066

Alternative size available: ADI-901-066A (5x96 wells)

Highly sensitive with an optional acetylation protocol which increases sensitivity >10-fold

High throughput format, results for up to 39 samples in duplicate in just 3 hours

Flexible assay, species independent

Easy-to-use pre-coated plate and liquid color-coded reagents reduce errors

Efficient and well-established sample handling protocols

Widely cited in peer-reviewed publications

Product Details：

Alternative Name: cyclic AMP

Sensitivity: Non-acetylated: 1.18 pmol/ml (range 0.78 - 200 pmol/ml)

Acetylated: 0.006 pmol/ml (range 0.078 - 20 pmol/ml)

Assay Time: 3 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include tissue culture.

Wavelength: 405 nm

Species reactivity: Species independent

Crossreactivity: cyclic AMP (100%)

AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%)

Use/Stability: Store all components at +4°C, except standard and conjugate at -20°C.

Shipping: Shipped on Blue Ice

Contents: GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine

ADI-900-066A cAMP直接ELISA试剂盒用于细胞内cAMP定量的灵敏（0.006 pmol/ml）比色试剂盒。这是一种比色竞争免疫分析试剂盒，用于定量测定在0.1M HCl中溶解的细胞或组织中的细胞内cAMP，该试剂盒用于停止内源性磷酸二酯酶活性并稳定释放的环AMP。然后在微量滴定板中直接分析0.1M HCl处理过的样品，无需提取、干燥和重构。乙酰化（包括试剂）使灵敏度提高10倍以上。在405 nm处读取吸光度。与竞争对手相比，大的信号背景比提供了*的灵敏度。该试剂盒可让你通过GPCR轻松评估腺苷酸环化酶的活性。

艾美捷ADI-900-066A cAMP直接ELISA试剂盒基本参数：

备选名称：循环AMP

灵敏度：非乙酰化：1.18 pmol/ml（范围0.78-200 pmol/ml）

乙酰化：0.006 pmol/ml（范围0.078-20 pmol/ml）

化验时间：3小时

应用：ELISA，比色检测

应用说明：用于定量测定任何物种的细胞裂解物和组织中的cAMP。引用的样本类型包括组织培养。

波长：405 nm

物种反应性：物种无关

交叉反应性：环状AMP（100%）

AMP、ATP、环状GMP、GMP、GTP、环状UMP、CTP（<0.001%）

使用/稳定性：在+4°C下储存所有成分，-20°C下标准和共轭物除外。

运输：蓝冰运输

内容：GxR IgG微量滴定板、缀合物、抗体、0.1M HCl、中和试剂、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液、乙酸酐、三乙胺

cAMP直接ELISA试剂盒文献参考：

1.骨桥蛋白缺失增强心肌细胞β2-肾上腺素能受体依赖性抗纤维化信号传导：C.M.Pollard等。国际分子科学杂志。20, 1396 (2019);

2.培养基中葡萄糖浓度升高通过cAMP/PKA依赖性途径减少LLC-PK1细胞中SGLT2的膜转运：S.Sunilkumar等人。美国生理学杂志。细胞生理学。316，C913（2019）；

3.在成年小鼠的实验模型中，亮-氨-酸-甘-氨-酸和肌肽二肽可预防由多次低剂量链脲佐菌素诱导的糖尿病：T.Vahdatpour等人。J、 糖尿病研究。10，1177（2019），应用：雄性BALB/c小鼠血液样本中的cAMP水平