中英文说明丨艾美捷RPMI-1640培养基含L-谷丨氨酰胺

RPMI-1640 was developed by Moore, et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Powder with uniform and free flowing appearance from pink to orange

Solubility Magenta, clear, complete

pH7.9±0.9

Endotoxin ≤ 1 unit/ml

For each liter, dissolve 10.4g in 900ml ddH2O, and stir until completely dissolved. Do not heat. If necessary, add 2g sodium bicarbonate with stirring. Adjust the pH value of the medium to 0.1-0.3 pH units lower than the required pH value. Add additional water to bring the solution to its final volume. Filter and disinfect with 0.22um membrane. Divide equally into sterile containers.

RPMI-1640广泛应用于哺乳动物、特殊造血细胞、正常或恶性增生的白细胞，杂交瘤细胞的培养，是目前应用十分广泛的培养基。主要用于悬浮细胞培养。其它像K-562、HL-60、Jurkat、Daudi、IM-9等成淋巴细胞、T细胞淋巴瘤细胞以及HCT-15上皮细胞等均可参考使用。

RPMI-1640培养基已用于培养人正常和肿瘤白细胞。当适当补充RPMI-1640时，已证明其广泛适用于支持多种类型的培养细胞的生长，包括72小时植物血凝素（PHA）刺激试验中的新鲜人淋巴细胞。

艾美捷RPMI-1640培养基L-谷丨氨酰胺含L-谷丨氨酰胺和25mMHEPES，不含碳丨酸氢钠，粉末形式。

英文名字：RPMI 1640, With L-glutamine and 25 mM HEPES. Without sodium bicarbonate.

外观：粉色至橙色均匀、自由流动的粉末

pH：7.9±0.9

内毒素：≤1EU/ml

等级：细胞培养等级

储存温度：室温/4℃

相关文献：

1. Moore, G.E., Gerner, R.E., Franklin, H.A., JAMA 199: 519-524 (1967). 2. Moore, G.E., Woods L.K., Tissue Culture Association Manual 3: 503-508. (1976). 3. Moore, G.E. Gerner, R.E., Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research 41-63. (1967, February). 4. Moore, G.E. Gerner, R.E., Kitamura,H., NY State Journal of Medicine 68: 2054-2060 (1968).