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中英文说明书丨Jackson正常兔血清

时间:2022/7/14阅读:158
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动物实验是医学科学发展的*学科家兔又是动物实验时的shou选动物为使动物实验资料和科学性更加完善接下来一起了解下艾美捷Jackson 正常兔血清

 

艾美捷Jackson 正常兔血清产品可应用于大多数免疫学实验,用作封闭液或空白对照.

 

艾美捷Jackson 正常兔血清使用

正常兔血清是以冻干粉形式提供的,本品需要先溶解配制成100%原液后再稀释使用。溶解方法:用5 mL 蒸馏水(dH2O)充分溶解冻干粉(如果不澄清,可对其离心处理),此时得到的即是100%原液。然后按照1:20的比例用缓冲液,如PBSPBST,稀释到需要的5%工作液。也可以根据自身实验条件,稀释到合适的工作液浓度。

 

艾美捷011-000-120正常兔血清化学性质

全名:正常兔血清丨Normal Rabbit Serum

Cat#011-000-120

规格:5ml

蛋白浓度(Protein concentration):60 mg/mL

缓冲液(Buffer):0.01 M磷酸钠,0.25 M氯化钠,pH 7.6

防腐剂(Preservative):0.05%die氮化钠

运输与保存方法:冰袋运输。冻干粉保存于2-8℃。

 

Information

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

 

Usage

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.


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