当前位置:武汉艾美捷科技有限公司>>技术文章>>中英文说明书丨Jackson正常兔血清
动物实验是医学科学发展的*学科。家兔又是动物实验时的shou选动物,为使动物实验资料和科学性更加完善,接下来一起了解下艾美捷Jackson 正常兔血清。
艾美捷Jackson 正常兔血清产品可应用于大多数免疫学实验,用作封闭液或空白对照.
艾美捷Jackson 正常兔血清使用:
正常兔血清是以冻干粉形式提供的,本品需要先溶解配制成100%原液后再稀释使用。溶解方法:用5 mL 蒸馏水(dH2O)充分溶解冻干粉(如果不澄清,可对其离心处理),此时得到的即是100%原液。然后按照1:20的比例用缓冲液,如PBS或PBST,稀释到需要的5%工作液。也可以根据自身实验条件,稀释到合适的工作液浓度。
艾美捷011-000-120正常兔血清化学性质:
全名:正常兔血清丨Normal Rabbit Serum
Cat#:011-000-120
规格:5ml
蛋白浓度(Protein concentration):60 mg/mL
缓冲液(Buffer):0.01 M磷酸钠,0.25 M氯化钠,pH 7.6
防腐剂(Preservative):0.05%die氮化钠
运输与保存方法:冰袋运输。冻干粉保存于2-8℃。
Information
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Usage
Physical State: Freeze-dried solid
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
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