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当前位置:北京方程嘉鸿科技有限公司>>ELISA试剂盒>>大鼠ELISA试剂盒>>白介素1可溶性受体Ⅱ(IL-1sRⅡ)

白介素1可溶性受体Ⅱ(IL-1sRⅡ)

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更新时间:2017-04-11 22:33:33浏览次数:112次

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产品简介

北京方程生物公司经营销售白介素1可溶性受体Ⅱ(IL-1sRⅡ)ELISA试剂盒,可以提供免费代测,送检赠京东卡活动正在进行中,详情咨询销售人员

详细介绍

CXCL16

试剂盒名称:白介素1可溶性受体Ⅱ(IL-1sRⅡ)ELISA试剂盒

英文名:Human CXC-chemokine ligand 16,CXCL16

 

品牌:BIOFINE
种属:大鼠ELISA试剂盒
检测波长:450nm
所需样本体积: 50-100ul
适用范围:仅供科研
保存及有效期:2-8℃,六个月,-20℃一年
检测目的:用于测定血清,血浆及相关液体白介素1可溶性受体Ⅱ(IL-1sRⅡ)含量。适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。
 

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.

 

白介素1可溶性受体Ⅱ(IL-1sRⅡ)elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度,损失越少,回收率越高,如果作标液1PPM,就是1毫克/升,而作出标准数据为0.99毫克/升,就是说你的回收率是99%,这个与真实成分有密切的关系,说明方法的准确度。比如水中总无机氯含量测定,样品水中含有无机氯20mg/L,取100mL被测水样品,加入0.1mL浓度为10mg/mL的含无机氯标准样品,测定时忽略体积变化,如果测定出样品中无机氯为2.98mg/L,则认为回收率为99%。

 

白介素1可溶性受体Ⅱ(IL-1sRⅡ)

骨髓抑制因子1(MPIF-1/CCL23)

氧化低密度脂蛋白抗体(OLAb)

磷脂酰肌醇抗体IgG/IgM(PI Ab-IgG/IgM)

*(PK)

γ谷氨酰半*合成酶(γ-ECS)

可溶性CD28(sCD28)

腺苷脱氨酶(ADA)

抗肝素PF4复合物抗体/HIT抗体(HIT)

沙眼衣原体(CT)

超敏前列腺特异性抗原(PSA-U S)

乳铁传递蛋白/乳铁蛋白(LF/LTF)

补体蛋白4(C4)

 

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