ELISA是以免疫学反应为基础，将抗原、抗体体的特异性反应与酶对底物的高效催化作用相结合起来的一种敏感性很高的试验技术。
小鼠内分泌腺来源的血管内皮生长因子 EG-VEGF是北京方程生物公司的优势产品，2017年买小鼠内分泌腺来源的血管内皮生长因子 EG-VEGF送好礼*活动火热进行中......
Pig Heat Shock 70kDa Protein 1B (HSPA1B) ELISA
ELISA results using S-OIV A neuraminidase antibody at 1 μg/ml to probe the immunogenic and the corresponding seasonal influenza A neuraminidase peptides at 50, 10, 2, and 0 ng/ml. Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[3] or West Nile Virus). It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.[4] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs. The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400-fold and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme. Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, for example, is established, and a sample that contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test.
1.2.2 抗体的产生 机体受抗原刺激后，B淋巴细胞产生相应的抗体。含有抗体的血清称为抗血清（antiserum）。每一系B细胞只产生针对某一抗原决定簇的抗体。如将多种抗原或含有多个抗原决定簇的抗原注入机体，则将由多系的B细胞产生相应的多种抗体，这些抗体均存在于免疫血清中。免疫测定中所用的抗血清一般用抗原免疫兔、羊或马制得。产生抗体的B细胞可在体外与繁殖力强的肿瘤细胞融合成杂交瘤细胞。将单个杂交瘤细胞分离，在体内或体外培养而分泌的抗体单克隆抗体（monoclonal antibody，McAb或Mab）。单克隆抗体仅针对一种抗原决定簇，具有很高的特异性。单克隆抗体通常用抗原免疫小鼠制备。将免疫的脾细胞（含产生抗体的B细胞）与小鼠肿瘤细胞融合，分离杂交瘤细胞，接种于小鼠腹腔，产生的腹水中含有浓度很高的单克隆抗体。1.3 抗原抗体反应1.3.1 可逆性 抗原与抗体结合形成抗原抗体复合物的过程是一种动态平衡，其反应式为：Ag+Ab→Ag·Ab。.抗体的亲和力（affinity）是抗原抗体间的固有结合力，可以平衡常数K表示：K=[Ag·Ab]／[Ag][Ab]。Ag·Ab的解离程度与K值有关。高亲和力抗体的抗原结合点与抗原的决定簇在空间构型上非常适合，两者结合牢固，不易解离。解离后的抗原或抗体均能保持原有的结构和活性，因此可用亲和层析法来提纯抗原或抗体。在抗血清中，特异性的IgG抗体仅占总IgG中的极小部分。用亲和层析法提取的特异性抗体，称为亲和层析纯抗体，应用于免疫测定中可得到更好的效果。1.3.2 zui适比例 在恒定量的抗体中加入递增量的抗原形成抗体复合物（沉淀）的量见图1-4。曲线的高峰部分是抗原抗体比例zui合适的范围，称为等价带（zone of equivalence）。在等价带前后分别为抗体过剩带和抗原过剩带。如果抗原或抗体极度过剩，则无沉淀物形成，在免疫测定中称为带现象（zone phenomenon）。抗体过量称为前带（prezone），抗原地过量称为后带（postzone）。在用免疫学方法测定抗原时，应使反应系统中有足够的抗体量，否则测得的量会小于实际含量，甚至出现假阴性。
猪G蛋白偶联受体109B(GPR109B)检测盒Pig G Protein Coupled Receptor 109B (GPR109B) ELISA
猪Ⅰ型前胶原羧基端原肽(PⅠCP)检测盒Pig Procollagen I C-Terminal Propeptide (PICP) ELISA
人胰岛素样生长因子2-mRNA结合蛋白2(IGF2BP2)测定盒Human Insulin Like Growth Factor 2 mRNA Binding Protein 2 (IGF2BP2) ELISA
人白介素35(IL35)测定盒Human Interleukin 35 (IL35) ELISA
猪泛素A52残留核糖体蛋白融合产物1(UBA52)检测盒Pig Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) ELISA
人血管内皮生长因子145(VEGF145)测定盒Human Vascular Endothelial Growth Factor 145 (VEGF145) ELISA
猪颗粒酶A(GZMA)检测盒Pig Granzyme A (GZMA) ELISA
人巨噬细胞移动抑制因子(MIF)测定盒Human Macrophage Migration Inhibitory Factor (MIF) ELISA
人Toll样受体4(TLR4)测定盒Human Toll Like Receptor 4 (TLR4) ELISA
人肝细胞核因子1β(HNF1β)测定盒Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA
人干扰素γ(IFNγ)测定盒Human Interferon Gamma (IFNg) ELISA
猪Asc型氨基酸转运蛋白1(ASC1)检测盒Pig Asc Type Amino Acid Transporter 1 (ASC1) ELISA
