试剂盒名称:小鼠心肌营养素1 CT-1
Pig Insulin Like Growth Factor 2 Receptor (IGF2R) ELISA
规格: 96T/48T
品牌:BIOFINE
种属:人ELISA试剂盒
检测波长:450nm
所需样本体积: 50-100ul
适用范围:仅供科研
保存及有效期:2-8℃，六个月，-20℃一年
检测目的:用于测定血清，血浆及相关液体小鼠心肌营养素1 CT-1含量。适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。
Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
另一片段类似Fc，随后被分解成小分子多肽，无生物活性。 IgM是由五个单体组成的五聚体，含10个重链和10个轻链，具有10个抗原结合价，由于空间位置的影响，只表现为五个抗原结合价。IgM分子量约为900000，IgG分子量约为150000。 机体被微生物感染后，先产生IgM抗体，然后产生IgG抗体。经过一段时间，IgM抗体量逐渐减少而消失，而IgG抗体可*存在，在疾病*后可持续数年之久。IgM抗体一般为保护性抗体，具有免疫性。因此IgM抗体的测定，对某些传染病如甲型肝炎有较高的临床诊断价值。
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