ELISA是以免疫学反应为基础，将抗原、抗体体的特异性反应与酶对底物的高效催化作用相结合起来的一种敏感性很高的试验技术。
猪肝配蛋白B2(EFNB2)检测盒是北京方程生物公司的优势产品，2017年买猪肝配蛋白B2(EFNB2)检测盒送好礼*活动火热进行中......
猪肝配蛋白B2(EFNB2)检测盒
Pig Ephrin B2 (EFNB2) ELISA
实验准备】：1. ELISA试剂盒及待测样本2. 酶标仪(450nm波长滤光片)3. 高精度移液器，EP管及一次性吸头4. 37℃恒温箱，双蒸水或去离子水5. 吸水纸
猪肝配蛋白B2(EFNB2)检测盒contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test. History Before the development of the ELISA, the only option for conducting an immunoassay was radioimmunoassay, a technique using radioactively-labeled antigens or antibodies. In radioimmunoassay, the radioactivity provides the signal, which indicates whether a specific antigen or antibody is present in the sample. Radioimmunoassay was first described in a paper by Rosalyn Sussman Yalow and Solomon Berson published in 1960.[5] Because radioactivity poses a potential health threat, a safer alternative was sought. A suitable alternative to radioimmunoassay would substitute a non-radioactive signal in place of the radioactive signal. When enzymes (such as peroxidase) react with appropriate substrates (such as ABTS or 3,3’,5,5’-Tetramethylbenzidine), a change in color occurs, which is used as a signal. However, the signal has to be associated with the presence of antibody or antigen, which is why the enzyme has to be linked to an appropriate antibody. This linking process was independently developed by Stratis Avrameas and G.B. Pierce.[6] Since it is necessary to remove any unbound antibody or antigen by washing, the antibody or antigen has to be fixed to the surface of the container; i.e., the immunosorbent has to be prepared. A technique to accomplish this was published by Wide and Jerker Porath in 1966.[7] In 1971, Peter Perlmann and Eva Engvall at Stockholm University in Sweden, and Anton Schuurs and Bauke van Weemen in The Netherlands independently published papers that synthesized this knowledge into methods to perform EIA/ELISA.[8][9]
猪肝配蛋白B2(EFNB2)检测盒
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