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紫外交联仪 Stratalinker UV 2400
特点:
为了使核苷酸固定在膜上,传统的方法是将膜置于真空烘箱中80度烘2小时,在紫外交联仪上仅需30-60秒
与真空烘焙相比,增加杂交信号强度
内部配备光度检测仪,可以补偿由于紫外灯泡老化造成的输出偏移
具自动交联、时间和能量三种模式供选择
带有显示时间、能量的显示器及设置面板
紫外交联仪 Stratalinker UV 2400
应用:
将核酸共价结合到固相支持物(如硝酸纤维素膜)上
Northem,Southern,dot及slot印迹分析
杂交芯片的制备
菌落、噬菌斑筛选,琼脂糖凝胶中DNA的切割
产生胸腺二聚体,用于部分限制性内切酶消化作图
紫外敏感性测试,用于鉴定recA宿主菌
样品紫外照射处理
诱导培养细胞的凋亡
照射波长:254nm(配8瓦254nm灯泡5个)
内部尺寸:47.7W/34.3D/16.8H
紫外交联仪 Stratalinker UV 2400
Stratagene UV Stratalinker 2400
The Stratagene UV Stratalinker 2400 crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. For optimal crosslinking performance, each Stratalinker UV crosslinker is equipped with aninternal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs. The Stratalinker UV crosslinker may be used for Northern, Southern, dot or slot blot analysis, colony or plaque screening, nicking of DNA in agarose gels prior to blotting, dimer formation to perform partial digests for rapid restriction mapping, UV sensitivity testing for host strain verification, and UV irradiation of PCR samples. The Stratalinker UV crosslinker may be used in the Autocrosslink,Time, or Energy modes.
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