BMPO (Spin trapping reagent) 自旋捕获剂

产品标签

BMPO；DEPMPO；EMPO；Spin trapping reagent 自旋捕获剂；电子顺磁共振波谱（EPR）；CAS: 387334-31-8；

产品信息

产品描述

BMPO（5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide） 是一种新型高效且高稳定型的硝酮自旋捕获剂（Nitrone Spin Trapping Reagent），由美国威斯康星医学院Kalyanaraman教授的实验室团队开发，非常适合用于特异性检测和鉴定体内外形成的硫自由基、羟基自由基（•OH）和超氧阴离子自由基（•O^2-），通过形成用电子顺磁共振波谱（EPR）可区分的加合物来测定^[1-2]。

BMPO具有以下特点：

①特异性高，半衰期长：其它的硝酮自旋捕获剂比如：DMPO，难以轻易区分超氧阴离子和羟基自由基，因产生的DMPO-超氧加合物（半衰期t_½=45s）瞬间衰减产生DMPO-羟基加合物。与最近开发的自旋捕获剂：DEPMPO和EMPO类似，BMPO-超氧加合物不会衰减生成羟基加合物，但是，BMPO-超氧加合物的半衰期最长（t_½=23min）。

②产品稳定性高：DEPMPO和EMPO是液体自旋捕获剂，通常被硝基氧杂质污染，且效期有限。BMPO是固体的环形硝酮，通过结晶以高度纯化的状态提供，能够保存更长的周期，且不用担心降解。

③更高的信噪比：BMPO衍生的加合物在各自的EPR光谱中具有更高的信噪比，使其更适合用于检测细胞悬浮液中的亚硫酸、羟基和甲基自由基。

④高水溶性：水溶性好，更利于水相体系自由基的研究，尤其是生物体系的自由基研究。

产品特性

1） CAS NO：387334-31-8

2） 化学名：3,4-dihydro-2-methyl-1,1-dimethylethyl ester-2H-pyrrole-2-carboxylic acid-1-oxide

3） 同义名：5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide; BocMPO;

4） 分子式：C₁₀H₁₇NO₃

5） 分子量：199.2

6） 纯度：≥98%

7） 外观：结晶或结晶性粉末

8） 溶解性：溶于水、PBS（pH 7.2, 10mg/ml）、DMSO（25mg/ml）、无水乙醇（25mg/ml）

保存与运输方法

保存：-20ºC干燥保存，2年有效。

运输：冰袋运输。

注意事项

为了您的安全和健康，请穿实验服并戴一次性手套操作。

参考文献

1. Zhao, H., Joseph, J., Zhang, H., et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: A relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic. Biol. Med. 31(5), 599-606 (2001).

2. Khan, N., Wilmont, C.M., Rosen, G.M., et al. Spin traps: In vitro toxicity and stability of radical adducts. Free Radical Biology & Medicine 34(11), 1473-1481 (2003).

应用示例（仅作参考）

1. 文献来源：Mitchell DG, Rosen GM, Tseitlin M, Symmes B, Eaton SS, Eaton GR. Use of rapid-scan EPR to improve detection sensitivity for spin-trapped radicals. Biophys J. 2013 Jul 16;105(2):338-42. doi: 10.1016/j.bpj.2013.06.005. PMID: 23870255; PMCID: PMC3714875. Edited by MKBIO

①氧化酶体系内超氧阴离子（O₂⋅−）生成的测定：Typically, xanthine oxidase (0.04 U/mL) was added to pH ∼7.4 sodium phosphate buffer (50 mM) containing DTPA (1 mM) and hypoxanthine (0.5–400 μM, final concentration) to achieve rates of O2⋅− formation that ranged from 0.1 to 6.0 μM/min. We estimated the superoxide formation rate by monitoring the SOD-inhibitable reduction of ferricytochrome c (80 μM) at room temperature. Spin trapping was performed by addition of 100 mM BMPO in pH ∼7.4 phosphate-buffered saline (PBS; 50 mM) containing 1 mM DTPA to the solution of hypoxanthine and xanthine oxidase to achieve a final BMPO concentration of 50 mM in the reaction mixture. EPR spectra were recorded 10 min after mixing reagents. The half-life of BMPO-OOH at ambient temperature is reported to be ∼23 min. Solutions for control experiments contained SOD (30 U/mL).

Figure 2 Comparison of CW and rapid-scan spectra of BMPO-OOH in solution with a O₂^⋅− production rate of 0.1 μM/min, recorded 10 min after mixing reagents. The O₂^⋅− was produced by a hypoxanthine/xanthine oxidase mixture. The concentration of BMPO-OOH is ∼0.3 μM. (A) CW spectrum obtained with 55 G sweep width, 0.75 G modulation amplitude, single 30 s scan, 15 ms conversion time, 10 ms time constant, and 20 mW (B₁ = 170 mG) microwave power. (B) Deconvolved rapid-scan spectrum obtained with 55 G scan width, 51 kHz scan frequency, and 53 mW (B₁ = 250 mG) microwave power. Segments consisting of 12 sinusoidal cycles were averaged 100 k times, with a total data acquisition time of ∼30 s.

2. 文献来源：Wang, Z., Zhang, Y., Ju, E. et al. Biomimetic nanoflowers by self-assembly of nanozymes to induce intracellular oxidative damage against hypoxic tumors. Nat Commun 9, 3334 (2018).

①缺氧下的ESR测定（O₂^•−_， •OH）：For O₂^•− detection, the pre-deoxidized PBS (pH 5.0, 25 mM) contained 25 mM BMPO, 20 μg mL⁻¹ MnO₂@PtCo nanoflowers, 100 μM H₂O₂, 50% DMSO was prepared. After incubation of 5 min, ESR spectra were recorded. For •OH detection, the pre-deoxidized PBS (pH 5.0, 25 mM) contained 25 mM BMPO, 20 μg mL⁻¹ MnO₂@PtCo nanoflowers, 100 μM H₂O₂, 0.25 U mL⁻¹ SOD was prepared. After incubation of 5 min, ESR spectra were recorded. The following instrument settings were used for collecting ESR spectra: 1 G field modulation, 100 G scan range, and 20 mW microwave power.

Fig f ESR spectra of BMPO/•OOH adducts from different groups in the hypoxic H₂O₂ (100 μM) condition upon addition of DMSO. g ESR spectra of BMPO/•OH adducts were collected from different samples in the hypoxic H₂O₂ (100 μM) condition upon addition of SOD. Data were presented as mean ± s.d. (n = 5).

相关产品

— —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信"的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。

BMPO 自旋捕获剂荧光探针

BMPO 自旋捕获剂荧光探针