ELISA试剂盒血红蛋白具有类过氧化物酶活性，可催化H2O2释放新生态氧，使邻甲联苯胺氧化发生颜色变化，由无色zui终变为蓝紫色。根据显色深浅与标准进行比较，可测出其含量。
实验方法
材料：
1．2g/L邻甲联苯胺溶液
2．1%H2O2
3．10%醋酸溶液
4．分光光度计
方法：ELISA试剂盒
抽取静脉血2-3ml，*抗凝，离心分离血浆。
3.计算公式
血浆游离Hb(mg/L)=测定管吸光度/标准管吸光度×100
实验结果计算
参考范围：0-40mg/L
注意事项ELISA试剂盒
1.一切容器避免血红蛋白污染；
2.采血要顺利，器皿要干燥，检测血浆要新鲜，及时分离，防止人为溶血；
3.邻联苯胺溶液具致癌性，检测时要小心，废液要妥善处理，以免污染；
4.双氧水要新鲜配置，浓度要准确。
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