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人慢性髓原白血病细胞

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产品简介

人慢性髓原白血病细胞描述:连续细胞株K-562由Lozzio从一位临终的53岁女性慢性骨髓性白血病患者的胸水中建立。 细胞被归入高度未分化的粒性白细胞类。 Anderson等对其表面膜性质的研究表明,K-562是一株人类红白血病细胞。 K-562细胞株在自然杀伤分析中广泛作为高敏感的体外受体。

详细介绍

细胞名称

K-562人慢性髓原白血病细胞

描述

连续细胞株K-562由Lozzio从一位临终的53岁女性慢性骨髓性白血病患者的胸水中建立。 细胞被归入高度未分化的粒性白细胞类。 Anderson等对其表面膜性质的研究表明,K-562是一株人类红白血病细胞。 K-562细胞株在自然杀伤分析中广泛作为高敏感的体外受体。 See Pross等将它用于NK细胞的体外详细检测,包括NK细胞活性的数学量化。 K-562母细胞是多能性,造血恶性细胞,它能自发分化成可识别的红血球祖细胞、粒性白细胞、单核细胞等。它是EBNA阴性的。

动物种别

性别

组织来源

骨髓;慢性骨髓性白血病(CML)

形态

淋巴母细胞

培养基和添加剂

IMDM培养基(GIBCO,货号12200036),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。

供应限制

A。仅供研究之用。

REFERENCE

Koeffler HP , Golde DW . Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765 Ortaldo JR , et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J. Natl. Cancer Inst. 59: 77-82, 1977. PubMed: 69036 Lozzio CB , Lozzio BB . Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. PubMed: 163658 Lozzio BB , Lozzio CB . Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk. Res. 3: 363-370, 1979. PubMed: 95026 Andersson LC , et al. K562--a human erythroleukemic cell line. Int. J. Cancer 23: 143-147, 1979. PubMed: 367973 Lozzio BB , et al. A multipotential leukemia cell line (K-562) of human origin. Proc. Soc. Exp. Biol. Med. 166: 546-550, 1981. PubMed: 7194480 Dimery IW , et al. Variation amongst K562 cell cultures. Exp. Hematol. 11: 601-610, 1983. PubMed: 6576909 Chan YJ , et al. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus. J. Virol. 70: 5312-5328, 1996. PubMed: 8764042 Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Gan W , Rhoads RE . Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663 Tiffany HL , et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842 Chan YJ , et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984 Nauseel WM , et al. Effect of the R569W missense mutation on the biosynthesis of myeloperoxidase. J. Biol. Chem. 271: 9546-9549, 1996. PubMed: 8621627 Grune T , et al. Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome. J. Biol. Chem. 271: 15504-15509, 1996. PubMed: 8663134 Jondal M , Pross H . Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. Int. J. Cancer 15: 596-605, 1975. PubMed: 806545 West WH , et al. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J. Immunol. 118: 355-361, 1977. PubMed: 299761 Pross HF , et al. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity. J. Clin. Immunol. 1: 51-63, 1981. PubMed: 7334070 Chen TR . Modal karyotype of human leukemia cell line, K562 (ATCC CCL 243). Cancer Genet. Cytogenet. 17: 55-60, 1985. PubMed: 3857109 Wu SQ , et al. Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-56. Leukemia 9: 858-862, 1995. PubMed: 7769849

中文名称

人慢性髓原白血病细胞


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