培养基和添加剂 | RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，80%；胎牛血清，20%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 |
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