培养基和添加剂 | DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，71.25%；F-12培养基(GIBCO,货号21700075，添加L-glutamine 300mg/L, NaHCO3 1.5g/L)，23.75%；添加14mM HEPES；优质胎牛血清，5%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 |
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