培养基和添加剂 | RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 |
REFERENCE | Science 229: 393-395, 1985. PubMed: 3839317 Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 EMBO J. 2: 643-648, 1983. PubMed: 6641712 YUAN Chong Gang et al., Acta Biologiae Experimentalis Sinica 35(2):98-102,2002 Prog. Biochem. Biophys. 29(4):621-623, 2002 Acta Universitatis Medicinalis Nanjing 22(2):116-118,2002 Acta Universitatis Medicinalis Nanjing(Natural Science) 22(4):271-273,2002 Acta Universitatis Medicinalis Nanjing(Natural Science) 22(5):425-426,2002 Prog. Biochem. Biophys. 30(6):960-964,2003 Fudan Univ J Med Sci 30(5):422-425,2003 Acta Academae Medicinae Miltaris Tertiae 25(5):426,2003 Acta Academae Medicinae Miltaris Tertiae 25(14):1217-1219,2003 Acta Academae Medicinae Miltaris Tertiae 25(14):1220-1222,2003 Acta Academae Medicinae Miltaris Tertiae 25(24):2173-2176,2003 |
