产品英文名： Human N-terminal procollagen Ⅳ propeptide,PⅣNP ELISA Kit ，人Ⅳ型前胶原氨基末端肽(PⅣNP)ELISA试剂盒市场常用名： 人Ⅳ型前胶原氨基末端肽(PⅣNP)ELISA检测试剂盒
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elisa试剂盒总需要时间来保温，是因为ELISA属固相免疫测定，抗原、抗体的结合只在固相表面上发生。以抗体包被的夹心法为例，加入板孔中的标本，其中的抗原并不是都有均等的和固相抗结合的机会，只有zui贴近孔壁的一层溶液中的抗原直接与抗体接触。这是一个逐步平衡的过程，因此需经扩散才能达到反应的终点。
在人Ⅳ型前胶原氨基末端肽(PⅣNP)ELISA试剂盒中一般有两次抗原抗体反应，即加标本和加酶结合物后。抗原抗体反应的完成需要有一定的温度和时间，这一保温过程称为温育，有人称之为孵育，在ELISA试剂盒中似不恰当。
Bos taurus; Bovine (Cattle) 白介素16(IL16)酶联免疫吸附试剂盒 ELISA Kit for Interleukin 16 (IL16) 48T/96T
Sus scrofa; Porcine (Pig) 白介素16(IL16)酶联免疫吸附试剂盒 ELISA Kit for Interleukin 16 (IL16) 48T/96T
Bos taurus; Bovine (Cattle) 白介素17(IL17)酶联免疫吸附试剂盒 ELISA Kit for Interleukin 17 (IL17) 48T/96T
Equus caballus; Equine (Horse) 白介素17(IL17)酶联免疫吸附试剂盒 ELISA Kit for Interleukin 17 (IL17) 48T/96T
Homo sapiens (Human) 成纤维细胞生长因子受体1(FGFR1)酶联免疫吸附试剂盒 ELISA Kit for Fibroblast Growth Factor Receptor 1 (FGFR1) 48T/96T
Rhesus monkey (Simian) 转化生长因子β2(TGFβ2)酶联免疫吸附试剂盒 ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) 48T/96T
Mus musculus (Mouse) 成纤维细胞生长因子23(FGF23)酶联免疫吸附试剂盒 ELISA Kit for Fibroblast Growth Factor 23 (FGF23) 48T/96T
Rattus norvegicus (Rat) 胰岛素样生长因子结合蛋白6(IGFBP6)酶联免疫吸附试剂盒 ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6) 48T/96T
Rattus norvegicus (Rat) 胰岛素样生长因子结合蛋白5(IGFBP5)酶联免疫吸附试剂盒 ELISA Kit for Insulin Like Growth Factor Binding Protein 5 (IGFBP5) 48T/96T
Bos taurus; Bovine (Cattle) 转化生长因子β2(TGFβ2)酶联免疫吸附试剂盒 ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) 48T/96T
Capra hircus; Caprine (Goat) 转化生长因子β2(TGFβ2)酶联免疫吸附试剂盒 ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) 48T/96T
Homo sapiens (Human) 淋巴细胞胞浆蛋白1(LCP1)酶联免疫吸附试剂盒 ELISA Kit for Lymphocyte Cytosolic Protein 1 (LCP1) 48T/96T
Mus musculus (Mouse) 环胍氨酸肽(CCP)酶联免疫吸附试剂盒 ELISA Kit for Cyclical Citrullinated Peptide (CCP) 48T/96T
Mus musculus (Mouse) 软骨寡聚基质蛋白(COMP)酶联免疫吸附试剂盒 ELISA Kit for Cartilage Oligomeric Matrix Protein (COMP) 48T/96T
Rattus norvegicus (Rat) 线粒体乙醛脱氢酶(ALDM)酶联免疫吸附试剂盒 ELISA Kit for Aldehyde Dehydrogenase, Mitochondrial (ALDM) 48T/96T
Homo sapiens (Human) 脑型肌酸激酶(CKB)酶联免疫吸附试剂盒 ELISA Kit for Creatine Kinase, Brain (CKB) 48T/96T
Cavia (Guinea pig ) 胰岛素样生长因子1(IGF1)酶联免疫吸附试剂盒 ELISA Kit for Insulin Like Growth Factor 1 (IGF1) 48T/96T
General *4(VD4)酶联免疫吸附试剂盒 ELISA Kit for Vitamin D4 (VD4) 48T/96T
Bos taurus; Bovine (Cattle) 脂联素(ADP)酶联免疫吸附试剂盒 ELISA Kit for Adiponectin (ADP) 48T/96T
Bos taurus; Bovine (Cattle) 内脂素(VF)酶联免疫吸附试剂盒 ELISA Kit for Visfatin (VF) 48T/96T
Homo sapiens (Human) 血小板衍生生长因子AA(PDGFAA)酶联免疫吸附试剂盒 ELISA Kit for Plaet Derived Growth Factor AA (PDGFAA) 48T/96T
Sus scrofa; Porcine (Pig) 主要碱性蛋白(MBP)酶联免疫吸附试剂盒 ELISA Kit for Major Basic Protein (MBP) 48T/96T
Bos taurus; Bovine (Cattle) 肾上腺髓质素(ADM)酶联免疫吸附试剂盒 ELISA Kit for Adrenomedullin (ADM) 48T/96T
Sus scrofa; Porcine (Pig) 肾上腺髓质素(ADM)酶联免疫吸附试剂盒 ELISA Kit for Adrenomedullin (ADM) 48T/96T
Bos taurus; Bovine (Cattle) 干扰素β(IFNβ)酶联免疫吸附试剂盒 ELISA Kit for Interferon Beta (IFNb) 48T/96T
Canis familiaris; Canine (Dog) 干扰素β(IFNβ)酶联免疫吸附试剂盒 ELISA Kit for Interferon Beta (IFNb) 48T/96T
Mus musculus (Mouse) 磷脂酰肌醇蛋白聚糖4(GPC4)酶联免疫吸附试剂盒 ELISA Kit for Glypican 4 (GPC4) 48T/96T
Mus musculus (Mouse) 干扰素调节因子3(IRF3)酶联免疫吸附试剂盒 ELISA Kit for Interferon Regulatory Factor 3 (IRF3) 48T/96T
Homo sapiens (Human) 细胞外基质蛋白1(ECM1)酶联免疫吸附试剂盒 ELISA Kit for Extracellular Matrix Protein 1 (ECM1) 48T/96T
Equus caballus; Equine (Horse) 肾上腺髓质素(ADM)酶联免疫吸附试剂盒 ELISA Kit for Adrenomedullin (ADM) 48T/96T
Rhesus monkey (Simian) 干扰素β(IFNβ)酶联免疫吸附试剂盒 ELISA Kit for Interferon Beta (IFNb) 48T/96T
Canis familiaris; Canine (Dog) 白介素1受体拮抗剂(IL1RA)酶联免疫吸附试剂盒 ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) 48T/96T
Capra hircus; Caprine (Goat) 白介素1受体拮抗剂(IL1RA)酶联免疫吸附试剂盒 ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) 48T/96T
Equus caballus; Equine (Horse) 白介素1受体拮抗剂(IL1RA)酶联免疫吸附试剂盒 ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) 48T/96T
Oryctolagus cuniculus (Rabbit) 白介素1受体拮抗剂(IL1RA)酶联免疫吸附试剂盒 ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) 48T/96T
Equus caballus; Equine (Horse) 干扰素β(IFNβ)酶联免疫吸附试剂盒 ELISA Kit for Interferon Beta (IFNb) 人Ⅳ型前胶原氨基末端肽(PⅣNP)ELISA试剂盒 48T/96T
Rattus norvegicus (Rat) 基质金属蛋白酶11(MMP11)酶联免疫吸附试剂盒 ELISA Kit for Matrix Metalloproteinase 11 (MMP11) 48T/96T
保温的方式除有的ELISA仪器附有特制的电热块外，一般均采用水浴，可将ELISA试剂盒板置于水浴箱中，ELISA板底应贴着水面，使温度迅速平衡。为避免蒸发，板上应加盖，也可用塑料贴封纸或保鲜膜覆盖板孔，此时可让反应板漂浮在水面上。若用保温箱，ELISA试剂盒应放在湿盒内，湿盒要选用传热性良好的材料如金属等，在盒底垫湿的纱布，zui后将ELISA板放在湿纱布上。湿盒应先放在保温箱中预温至规定的温度，特别是在气温较低的时候更应如此。无论是水浴还是湿盒温育，反应板均不宜叠放，以保证各板的温度都能迅速平衡。室温温育的反应，操作时的室温应严格限制在规定的范围内，标准室温温度是指20～25℃，但具体操作时可根据说明书的要求控制温育。室温温育时，人Ⅳ型前胶原氨基末端肽(PⅣNP)ELISA试剂盒只要平置于操作台上即可。应注意温育的温度和时间应按规定力求准确。为保证这一点，一个人操作时，一次不宜多于两块板同时测定。
