产品英文名： Human melanoma ELISA Kit ，人黑色素瘤ELISA试剂盒市场常用名： 人黑色素瘤ELISA检测试剂盒
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elisa试剂盒总需要时间来保温，是因为ELISA属固相免疫测定，抗原、抗体的结合只在固相表面上发生。以抗体包被的夹心法为例，加入板孔中的标本，其中的抗原并不是都有均等的和固相抗结合的机会，只有zui贴近孔壁的一层溶液中的抗原直接与抗体接触。这是一个逐步平衡的过程，因此需经扩散才能达到反应的终点。
在人黑色素瘤ELISA试剂盒中一般有两次抗原抗体反应，即加标本和加酶结合物后。抗原抗体反应的完成需要有一定的温度和时间，这一保温过程称为温育，有人称之为孵育，在ELISA试剂盒中似不恰当。
Homo sapiens (Human) 多肽-N-乙酰氨基半乳糖转移酶11(GALNT11)酶联免疫试剂盒 ELISA Kit for Polypeptide-N-Acetylgalactosaminyltransferase 11 (GALNT11) 48T/96T
Homo sapiens (Human) 核糖核酸酶A11(RNASE11)酶联免疫试剂盒 ELISA Kit for Ribonuclease A11 (RNASE11) 48T/96T
Homo sapiens (Human) 突触融合蛋白19(STX19)酶联免疫试剂盒 ELISA Kit for Syntaxin 19 (STX19) 48T/96T
Homo sapiens (Human) Vertnin蛋白(VRTN)酶联免疫试剂盒 ELISA Kit for Vertnin (VRTN) 48T/96T
Homo sapiens (Human) 脂肪酶J(LIPJ)酶联免疫试剂盒 ELISA Kit for Lipase J (LIPJ) 48T/96T
Homo sapiens (Human) 核糖核酸酶A12(RNASE12)酶联免疫试剂盒 ELISA Kit for Ribonuclease A12 (RNASE12) 48T/96T
Homo sapiens (Human) 核仁蛋白11(1)酶联免疫试剂盒 ELISA Kit for Nucleolar Protein 11 (1) 48T/96T
Homo sapiens (Human) *蛋白酶抑制蛋白E3(SERPINE3)酶联免疫试剂盒 ELISA Kit for Serpin E3 (SERPINE3) 48T/96T
Homo sapiens (Human) 核糖核酸酶A13(RNASE13)酶联免疫试剂盒 ELISA Kit for Ribonuclease A13 (RNASE13) 48T/96T
Homo sapiens (Human) REST辅抑制因子3(RCOR3)酶联免疫试剂盒 ELISA Kit for REST Corepressor 3 (RCOR3) 48T/96T
Homo sapiens (Human) REST辅抑制因子2(RCOR2)酶联免疫试剂盒 ELISA Kit for REST Corepressor 2 (RCOR2) 48T/96T
Homo sapiens (Human) 减数分裂重组蛋白5(MEI5)酶联免疫试剂盒 ELISA Kit for Meiotic Recombination Protein 5 (MEI5) 48T/96T
Homo sapiens (Human) 组织蛋白酶L3(CTSL3)酶联免疫试剂盒 ELISA Kit for Cathepsin L3 (CTSL3) 48T/96T
Homo sapiens (Human) 烷基甘油单加氧酶(AGMO)酶联免疫试剂盒 ELISA Kit for Alkylglycerol Monooxygenase (AGMO) 48T/96T
Homo sapiens (Human) 细胞周期素I2(CCNI2)酶联免疫试剂盒 ELISA Kit for Cyclin I2 (CCNI2) 48T/96T
Homo sapiens (Human) 血管紧张素1-9(Ang1-9)酶联免疫试剂盒 ELISA Kit for Angiotensin 1-9 (Ang1-9) 48T/96T
Homo sapiens (Human) 半乳糖凝集素9B(GAL9B)酶联免疫试剂盒 ELISA Kit for Galectin 9B (GAL9B) 48T/96T
Homo sapiens (Human) Sesquipedalian 1蛋白(Ses1)酶联免疫试剂盒 ELISA Kit for Sesquipedalian 1 (Ses1) 48T/96T
Homo sapiens (Human) *蛋白酶31(PRSS31)酶联免疫试剂盒 ELISA Kit for Protease, Serine 31 (PRSS31) 48T/96T
Homo sapiens (Human) 半乳糖凝集素9C(GAL9C)酶联免疫试剂盒 ELISA Kit for Galectin 9C (GAL9C) 48T/96T
Homo sapiens (Human) 耳石蛋白1(OTOL1)酶联免疫试剂盒 ELISA Kit for Otolin 1 (OTOL1) 48T/96T
Homo sapiens (Human) 半乳糖凝集素7B(GAL7B)酶联免疫试剂盒 ELISA Kit for Galectin 7B (GAL7B) 48T/96T
Homo sapiens (Human) 封闭蛋白25(CLDN25)酶联免疫试剂盒 ELISA Kit for Claudin 25 (CLDN25) 48T/96T
Homo sapiens (Human) 干扰素α2(IFNα2)酶联免疫试剂盒 ELISA Kit for Interferon Alpha 2 (IFNa2) 48T/96T
Homo sapiens (Human) 核糖核酸酶P(RNASEP)酶联免疫试剂盒 ELISA Kit for Ribonuclease P (RNASEP) 48T/96T
Homo sapiens (Human) 核糖核酸酶H(RNASEH)酶联免疫试剂盒 ELISA Kit for Ribonuclease H (RNASEH) 48T/96T
Homo sapiens (Human) 输入蛋白8(IPO8)酶联免疫试剂盒 ELISA Kit for Importin 8 (IPO8) 48T/96T
Homo sapiens (Human) 酪胺酸酶(TYR)酶联免疫试剂盒 ELISA Kit for Tyrosinase (TYR) 48T/96T
Homo sapiens (Human) Nischarin蛋白(NISCH)酶联免疫试剂盒 ELISA Kit for Nischarin (NISCH) 48T/96T
Homo sapiens (Human) 眼皮肤白化病Ⅱ蛋白(OCA2)酶联免疫试剂盒 ELISA Kit for Oculocutaneous Albinism II (OCA2) 48T/96T
Homo sapiens (Human) 转换蛋白1(TNP1)酶联免疫试剂盒 ELISA Kit for Transition Protein 1 (TNP1) 48T/96T
Homo sapiens (Human) 蛛毒素受体3(LPHN3)酶联免疫试剂盒 ELISA Kit for Latrophilin 3 (LPHN3) 48T/96T
Homo sapiens (Human) 下丘泌素受体2(HCRTR2)酶联免疫试剂盒 ELISA Kit for Hypocretin Receptor 2 (HCRTR2) 48T/96T
Homo sapiens (Human) 下丘泌素受体1(HCRTR1)酶联免疫试剂盒 ELISA Kit for Hypocretin Receptor 1 (HCRTR1) 48T/96T
Homo sapiens (Human) 食欲素(OX)酶联免疫试剂盒 ELISA Kit for Orexin (OX) 48T/96T
Homo sapiens (Human) 组织蛋白酶A(CTSA)酶联免疫试剂盒 ELISA Kit for Cathepsin A (CTSA) 48T/96T
Homo sapiens (Human) 蛛毒素受体2(LPHN2)酶联免疫试剂盒 ELISA Kit for Latrophilin 2 (LPHN2) 48T/96T
Homo sapiens (Human) *受体1(SSTR1)酶联免疫试剂盒 ELISA Kit for Somatostatin Receptor 1 (SSTR1) 48T/96T
Homo sapiens (Human) 突触素(SYP)酶联免疫试剂盒 ELISA Kit for Synaptophysin (SYP) 48T/96T
Homo sapiens (Human) 滋养层特异性糖蛋白(TPBG)酶联免疫试剂盒 ELISA Kit for Trophoblast Glycoprotein (TPBG) 48T/96T
Homo sapiens (Human) 桥粒芯糖蛋白3(DSG3)酶联免疫试剂盒 ELISA Kit for Desmoglein 3 (DSG3) 48T/96T
Homo sapiens (Human) 血小板亲和蛋白2(PKP2)酶联免疫试剂盒 ELISA Kit for Plakophilin 2 (PKP2) 48T/96T
Homo sapiens (Human) 盐皮质激素受体(MR)酶联免疫试剂盒 ELISA Kit for Mineralocorticoid Receptor (MR) 48T/96T
Homo sapiens (Human) 角蛋白6B(KRT6B)酶联免疫试剂盒 ELISA Kit for Keratin 6B (KRT6B) 48T/96T
Homo sapiens (Human) 角蛋白5(KRT5)酶联免疫试剂盒 ELISA Kit for Keratin 5 (KRT5) 48T/96T
Homo sapiens (Human) 角蛋白3(KRT3)酶联免疫试剂盒 ELISA Kit for Keratin 3 (KRT3) 48T/96T
Homo sapiens (Human) 蜗管蛋白(COIL)酶联免疫试剂盒 ELISA Kit for Coilin (COIL) 48T/96T
Homo sapiens (Human) 角蛋白15(KRT15)酶联免疫试剂盒 ELISA Kit for Keratin 15 (KRT15) 48T/96T
Homo sapiens (Human) 角蛋白12(KRT12)酶联免疫试剂盒 ELISA Kit for Keratin 12 (KRT12) 48T/96T
Homo sapiens (Human) 角蛋白7(KRT7)酶联免疫试剂盒 ELISA Kit for Keratin 7 (KRT7) 48T/96T
Homo sapiens (Human) ADP核糖转移酶4(ART4)酶联免疫试剂盒 ELISA Kit for ADP Ribosyltransferase 4 (ART4) 48T/96T
Homo sapiens (Human) 拓扑异构酶Ⅱ(TOP2)酶联免疫试剂盒 ELISA Kit for Topoisomerase II (TOP2) 48T/96T
Homo sapiens (Human) 谷氨氨肽酶(GluAP)酶联免疫试剂盒 ELISA Kit for Glutamyl Aminopeptidase (GluAP) 48T/96T
Homo sapiens (Human) 前-B-淋巴细胞基因1(VPREB1)酶联免疫试剂盒 ELISA Kit for Pre-B-Lymphocyte Gene 1 (VPREB1) 48T/96T
保温的方式除有的ELISA仪器附有特制的电热块外，一般均采用水浴，可将ELISA试剂盒板置于水浴箱中，ELISA板底应贴着水面，使温度迅速平衡。为避免蒸发，板上应加盖，也可用塑料贴封纸或保鲜膜覆盖板孔，此时可让反应板漂浮在水面上。若用保温箱，ELISA试剂盒应放在湿盒内，湿盒要选用传热性良好的材料如金属等，在盒底垫湿的纱布，zui后将ELISA板放在湿纱布上。湿盒应先放在保温箱中预温至规定的温度，特别是在气温较低的时候更应如此。无论是水浴还是湿盒温育，反应板均不宜叠放，以保证各板的温度都能迅速平衡。室温温育的反应，操作时的室温应严格限制在规定的范围内，标准室温温度是指20～25℃，但具体操作时可根据说明书的要求控制温育。室温温育时，只要平置于操作台上即可。应注意温育的温度和时间应按规定力求准确。为保证这一点，一个人操作时，一次不宜多于两块板同时测定。
