产品英文名： Human anti-TNF-alpha autoantibody ELISA kit ，人抗TNF-α自身抗体ELISA试剂盒市场常用名： 人抗TNF-α自身抗体ELISA检测试剂盒
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elisa试剂盒总需要时间来保温，是因为ELISA属固相免疫测定，抗原、抗体的结合只在固相表面上发生。以抗体包被的夹心法为例，加入板孔中的标本，其中的抗原并不是都有均等的和固相抗结合的机会，只有zui贴近孔壁的一层溶液中的抗原直接与抗体接触。这是一个逐步平衡的过程，因此需经扩散才能达到反应的终点。
在人抗TNF-α自身抗体ELISA试剂盒中一般有两次抗原抗体反应，即加标本和加酶结合物后。抗原抗体反应的完成需要有一定的温度和时间，这一保温过程称为温育，有人称之为孵育，在ELISA试剂盒中似不恰当。
Homo sapiens (Human) 白血病抑制因子(LIF)酶联免疫试剂盒 ELISA Kit for Leukemia Inhibitory Factor (LIF) 48T/96T
Homo sapiens (Human) 抗SSA/Ro抗体(Anti-Ro)酶联免疫试剂盒 ELISA Kit for Anti-SSA/Ro Antibody (Anti-Ro) 48T/96T
Homo sapiens (Human) 抗单链DNA抗体(Anti-ssDNA)酶联免疫试剂盒 ELISA Kit for Anti-Single Stranded DNA Antibody (Anti-ssDNA) 48T/96T
Homo sapiens (Human) α-胞衬蛋白(SPTAN1)酶联免疫试剂盒 ELISA Kit for Alpha-Fodrin (SPTAN1) 48T/96T
Homo sapiens (Human) 嗜中性粒细胞胞浆因子2(NCF2)酶联免疫试剂盒 ELISA Kit for Neutrophil Cytosolic Factor 2 (NCF2) 48T/96T
Homo sapiens (Human) 促甲状腺素(TSH)酶联免疫试剂盒 ELISA Kit for Thyroid Stimulating Hormone (TSH) 48T/96T
Rattus norvegicus (Rat) 促甲状腺素(TSH)酶联免疫试剂盒 ELISA Kit for Thyroid Stimulating Hormone (TSH) 48T/96T
Mus musculus (Mouse) 促甲状腺素(TSH)酶联免疫试剂盒 ELISA Kit for Thyroid Stimulating Hormone (TSH) 48T/96T
Homo sapiens (Human) 抗精子抗体(AsAb)酶联免疫试剂盒 ELISA Kit for Anti-Sperm Antibody (AsAb) 48T/96T
Homo sapiens (Human) 抗子宫内膜抗体(Anti-EMAb)酶联免疫试剂盒 ELISA Kit for Anti-Endometrium Antibody (Anti-EMAb) 48T/96T
Oryctolagus cuniculus (Rabbit) 脂联素(ADP)酶联免疫试剂盒 ELISA Kit for Adiponectin (ADP) 48T/96T
Oryctolagus cuniculus (Rabbit) 肿瘤坏死因子α(TNFα)酶联免疫试剂盒 ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) 48T/96T
Sus scrofa; Porcine (Pig) 表面活性物质关联蛋白A(SPA)酶联免疫试剂盒 ELISA Kit for Surfactant Associated Protein A (SPA) 48T/96T
Oryctolagus cuniculus (Rabbit) 转化生长因子β1(TGFβ1)酶联免疫试剂盒 ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) 48T/96T
General 组胺(HIS)酶联免疫试剂盒 ELISA Kit for Histamine (HIS) 48T/96T
Canis familiaris; Canine (Dog) 内皮素1(EDN1)酶联免疫试剂盒 ELISA Kit for Endothelin 1 (EDN1) 48T/96T
Homo sapiens (Human) *2(VD2)酶联免疫试剂盒 ELISA Kit for Vitamin D2 (VD2) 48T/96T
Homo sapiens (Human) *3(VD3)酶联免疫试剂盒 ELISA Kit for Vitamin D3 (VD3) 48T/96T
General *12(VB12)酶联免疫试剂盒 ELISA Kit for Vitamin B12 (VB12) 48T/96T
Rattus norvegicus (Rat) 速激肽受体2(TACR2)酶联免疫试剂盒 ELISA Kit for Tachykinin Receptor 2 (TACR2) 48T/96T
Mus musculus (Mouse) 速激肽受体2(TACR2)酶联免疫试剂盒 ELISA Kit for Tachykinin Receptor 2 (TACR2) 48T/96T
Oryctolagus cuniculus (Rabbit) 速激肽受体2(TACR2)酶联免疫试剂盒 ELISA Kit for Tachykinin Receptor 2 (TACR2) 48T/96T
Homo sapiens (Human) 肾损伤分子1(Kim1)酶联免疫试剂盒 ELISA Kit for Kidney Injury Molecule 1 (Kim1) 48T/96T
Rattus norvegicus (Rat) 肾损伤分子1(Kim1)酶联免疫试剂盒 ELISA Kit for Kidney Injury Molecule 1 (Kim1) 48T/96T
Mus musculus (Mouse) 肾损伤分子1(Kim1)酶联免疫试剂盒 ELISA Kit for Kidney Injury Molecule 1 (Kim1) 48T/96T
Mus musculus (Mouse) C-型钠尿肽(CNP)酶联免疫试剂盒 ELISA Kit for C-Type Natriuretic Peptide (CNP) 48T/96T
Rattus norvegicus (Rat) C-型钠尿肽(CNP)酶联免疫试剂盒 ELISA Kit for C-Type Natriuretic Peptide (CNP) 48T/96T
Homo sapiens (Human) *(PP)酶联免疫试剂盒 ELISA Kit for Pepsin (PP) 48T/96T
Homo sapiens (Human) 血小板衍生生长因子BB(PDGFBB)酶联免疫试剂盒 ELISA Kit for Plaet Derived Growth Factor BB (PDGFBB) 48T/96T
Rattus norvegicus (Rat) 血小板衍生生长因子B(PDGFB)酶联免疫试剂盒 ELISA Kit for Plaet Derived Growth Factor Subunit B (PDGFB) 48T/96T
Homo sapiens (Human) 表面活性物质关联蛋白J(SPJ)酶联免疫试剂盒 ELISA Kit for Surfactant Associated Protein J (SPJ) 48T/96T
Mus musculus (Mouse) 50kDa核孔蛋白(NUP50)酶联免疫试剂盒 ELISA Kit for Nucleoporin 50kDa (NUP50) 48T/96T
Homo sapiens (Human) 肌球蛋白轻链2(MYL2)酶联免疫试剂盒 ELISA Kit for Myosin Light Chain 2, Regulatory, Cardiac (MYL2) 48T/96T
Homo sapiens (Human) 抗甲基乙二醛抗体(AMA)酶联免疫试剂盒 ELISA Kit for Anti-Methylglyoxal Antibody (AMA) 48T/96T
Homo sapiens (Human) 辅肌动蛋白α3(ACTN3)酶联免疫试剂盒 ELISA Kit for Actinin Alpha 3 (ACTN3) 48T/96T
Homo sapiens (Human) 肌球蛋白轻链1(MYL1)酶联免疫试剂盒 ELISA Kit for Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) 48T/96T
Homo sapiens (Human) 肌球蛋白轻链激酶(MYLK)酶联免疫试剂盒 ELISA Kit for Myosin Light Chain Kinase (MYLK) 48T/96T
Mus musculus (Mouse) 白血病抑制因子(LIF)酶联免疫试剂盒 ELISA Kit for Leukemia Inhibitory Factor (LIF)人抗TNF-α自身抗体ELISA试剂盒 48T/96T
Homo sapiens (Human) L选择素(SELL)酶联免疫试剂盒 ELISA Kit for Selectin, Leukocyte (SELL) 48T/96T
保温的方式除有的ELISA仪器附有特制的电热块外，一般均采用水浴，可将ELISA试剂盒板置于水浴箱中，ELISA板底应贴着水面，使温度迅速平衡。为避免蒸发，板上应加盖，也可用塑料贴封纸或保鲜膜覆盖板孔，此时可让反应板漂浮在水面上。若用保温箱，ELISA试剂盒应放在湿盒内，湿盒要选用传热性良好的材料如金属等，在盒底垫湿的纱布，zui后将ELISA板放在湿纱布上。湿盒应先放在保温箱中预温至规定的温度，特别是在气温较低的时候更应如此。无论是水浴还是湿盒温育，反应板均不宜叠放，以保证各板的温度都能迅速平衡。室温温育的反应，操作时的室温应严格限制在规定的范围内，标准室温温度是指20～25℃，但具体操作时可根据说明书的要求控制温育。室温温育时，人抗TNF-α自身抗体ELISA试剂盒只要平置于操作台上即可。应注意温育的温度和时间应按规定力求准确。为保证这一点，一个人操作时，一次不宜多于两块板同时测定。
