β-Actin抗体【Anti-β-Actin抗体】
Catalog Number :RS-0061R
β-Actin抗体【Anti-β-Actin抗体】
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
β-Actin抗体【Anti-β-Actin抗体】
Background: All eukaryotic cells express Actin, which often constitutes as much
as 50% of total cellular protein. Actin filaments can form both stable and labile
structures and are crucial components of microvilli and the contractile apparatus of
muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene,
higher eukaryotes have several isoforms encoded by a family of genes. At least six
types of Actin are present in mammalian tissues and fall into three classes. α Actin
expression is limited to various types of muscle, whereas β and γ are the principle
constituents of filaments in other tissues. Members of the small GTPase family
regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin
stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the
plasma membrane and Cdc42 stimulates formation of filopodia.
.Specificity : Anti-β-Actinis a Rabbit polyclonal antibody
specific for β-Actinof Human,
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 43 kDa
Application :
– WB=1:100-500
– ELISA=1:500-1000
– IP=1:20-100
– IHC-P=1:100-500
– IHC-F=1:100-500
– IF=1:100-500
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
