细胞表面趋化因子受体6抗体操作步骤

　　产品名称：细胞表面趋化因子受体6抗体，CCR-6/CD196抗体
　　相关标记： HRP  Biotin  Gold  RBITC  AP  FITC  Cy3  Cy5  Cy5.5  Cy7  PE  PE-Cy3  PE-CY5  PE-CY5.5  PE-CY7  APC  Alexa Fluor 350  Alexa Fluor 488  Alexa Fluor 555 Alexa Fluor 647
　　浓 度： 1mg/1ml
　　抗体来源： Rabbit or Mouse
　　克隆类型： polyclonal or monoclonal
　　产品类型 一抗
　　研究领域 肿瘤 信号转导 转录调节因子 细胞表面分子
　　蛋白分子量 predicted molecular weight: 41kDa
　　性 状 Lyophilized or Liquid
　　免 疫 原 KLH conjugated synthetic peptide derived from human CCR6 C-terminus
　　亚 型 IgG
　　纯化方法 affinity purified by Protein A
　　储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
　　产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

　　保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
　　Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

　　产品介绍 CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein coupled receptors. It is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha （MIP 3 alpha）. This receptor has been shown to be important for B lineage maturation and antigen driven B cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. CCR6 has been reported to be expressed in intestine, colon, liver, lymph node, spleen, lung, blood, umbilical cord, placenta, skin, and pancreas. It has also been reported to be expressed in lymphocytes, T cells, and B cells.

　　免疫组化结果分析：
　　1、首先要确定免疫组化技术是否合格，合格的染色结果应该是背景颜色浅淡或无着色，而阳性标志物清晰和定位明确。
　　切片中的正常组织可以作为-简便的对照来判断染色结果的可靠与否，例如用Vimentin抗体，间质成纤维细胞利血管壁平滑肌细胞胞浆应呈阳性。肯定为阳性的对照组织却呈阴性，待测组织的染色结果也就不可靠；这现象常是由于抗体失效或过度稀释、组织中抗原严重丢失等。
　　2、不仅要判断所检组织中有或无阳性标记物，还要注意阳性信号的分布部位和形态特征。
　　一般说来，阳性信号应与被测抗原所在的部位一致，大部分细胞抗原位于胞质内，其中亦有区别，有的阳性颗粒弥散于整个细胞的胞质，有的则呈斑块状局限于胞浆某处。还有些抗原同时分布于胞质和胞膜(如胃肠腺癌细胞的EMA)，或核与胞质(如S-100、HMB-45)。同一种抗原在不同类型的细胞可定位不同，因而分布的特点也有一定的鉴别意义。