细胞毒性T细胞抗原-4抗体

中文名称    细胞毒性T细胞抗原-4抗体    
别    名    CD 152; CD152; CD152 antigen; Celiac disease 3; CELIAC3; CTLA 4; CTLA-4; Cytotoxic T cell associated 4; Cytotoxic T lymphocyte associated 4; Cytotoxic T lymphocyte associated antigen 4; Cytotoxic T lymphocyte associated protein 4; Cytotoxic T lymphocyte associated serine esterase 4; Cytotoxic T lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; Cytotoxic T-lymphocyte-associated protein 4; GSE; IDDM12.      
Specific References  (1)     |     bs-10006R has been referenced in 1 publications.
[IF=4.12] Seike, Masahiro, et al. "Histamine suppresses regulatory T cells mediated by TGF‐β in murine chronic allergic contact dermatitis." Experimental Dermatology(2015).  IHC-F ;  Mouse.  
PubMed:25651189
研究领域    
抗体来源    Rabbit    
克隆类型    Polyclonal    
交叉反应    Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,     
产品应用    WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    21kDa    
细胞定位    细胞膜 细胞外基质     
性    状    Lyophilized or Liquid    
浓    度    1mg/ml    
免 疫 原    KLH conjugated synthetic peptide derived from human CTLA-4/CD152:75-170/223 <Extracellular>    
亚    型    IgG    
纯化方法    affinity purified by Protein A    
储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    
PubMed    PubMed    
产品介绍    background:
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
抗原抗体结合特点
 1）特异性：抗原分子只能与由它刺激所产生的抗体结合而起反应的专一性能；
 2）可逆性：是指Ag与相应Ab结合成IC后，在一定条件下可解离为游离抗原与抗体。
 3）阶段性：
 一阶段：特异性结合阶段 （①反应速度快，几秒钟或几分钟即可完成 ②不出现肉眼可见反应）；
 第二阶段：可见阶段（表现为凝集、沉淀、补体结合等反应 ①反应进行慢，需要几分钟、几十分钟或geng长②受电解质、温度、酸碱度等多种因素影响）。
4）比例性：（zui适比或等价点）是指抗原与抗体发生可见反应需遵循一定的量比关系，只有当二者浓度比例适当时，才出现可见反应。
抗原抗体反应影响因素：
（一）反应物自身的因素
5） 抗体：不同来源的抗体,反应性各有差异,抗体的浓度、特异性和亲和力都影响抗体抗原反应,为提高试验的可靠性,应选择高特异性、高亲和力的抗体作诊断试剂.等价带的宽窄也影响抗原抗体复合物的形成,单克隆抗体不适用于沉淀反应.
6）  抗原：抗原的理化性状、分子量、抗原决定簇的种类及数目均可影响反应结果.颗粒性抗原出现凝集反应,可溶性抗原出现沉淀反应,单价抗原与相应抗体结合不出现沉淀现象.
（二）反应环境条件
7） 酸碱度：抗原抗体反应必须在合适的pH环境中进行.蛋白质具有两性电离性质,因此每种蛋白质都有固定的等电点.抗原抗体反应一般在pH6～9进行,有补体参与的反应pH为7.7.4,pH过高或过低都将影响抗原与抗体反应.
8）  温度：在一定范围内,温度升高可加速分子运动,抗原与抗体碰撞机会增多,使反应加速.一般为15℃～40℃,常用的抗原抗体反应温度为37℃,温度如高于56℃,可导致已结合的抗原抗体再解离,甚至变性或破坏.每种试验都有其*的zui适反应温度要求.此外,适当振荡也可促进抗原抗体分子的接触,加速反应.