Mouse Anti-Nestin说明书 antibody
Catalog Number :RS-6065R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background: background:
There are a certain number of neural stem cells, which can be potentially differentiated into the neurons or neuroglia, in the adult mammalian brain. These neural precursors are located in the subependymal cell layer of the ventricle. Those precursors isolated from embryonic brain can be divided and differentiated into the neurons and glia in vitro and this is regulated by a series of growth-factor actions.
Function:
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Specificity :
–Anti-Nestin is a Mouse polyclonal antibody
–specific for Nestin of rat ,human
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 178 kDa
Application :
- WB=1:500-1000
- IHC-P=1:100-500
- IHC-F=1:100-500
- IF=1:50-200 ,not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
